Rabbit Recombinant Monoclonal SPARCL1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunocytochemistry-immunofluorescence-img418491.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639)")
![Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunoprecipitation-img418428.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639)")
![Western blot - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--western-blot-img418414.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639)")
![Flow Cytometry (Intracellular) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--flow-cytometry-intracellular-img418505.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418473.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (AB313639)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | IP | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Alternative names
Ecm2, Sc1, Sparcl1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1
Recommended products
Rabbit Recombinant Monoclonal SPARCL1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28135-54
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPARCL1 also known as Hevin or Secreted Protein Acidic and Rich in Cysteine-Like 1 is a protein with a mass of approximately 75 kDa. It is part of the SPARC (Secreted Protein Acidic and Rich in Cysteine) family of matricellular proteins. SPARCL1 is mainly expressed in various tissues including the heart brain lungs and skeletal muscle. This protein exhibits strong conservation across species hinting at its importance to fundamental biological processes.
- Biological function summary
SPARCL1 regulates cell-matrix interactions. It is not part of a protein complex but it interacts with other extracellular matrix components to influence cell adhesion and migration. SPARCL1 plays a role in normal homeostasis and is involved in the control of the extracellular matrix organization. It affects cellular behavior by modulating the activity of matrix metalloproteinases and influences the adhesion properties of cells by binding to components even in absence of complex formation.
- Pathways
SPARCL1 impacts cellular processes like tissue remodeling and inflammation. It operates within the ECM remodeling and focal adhesion pathways. In ECM remodeling related proteins such as MMPs (Matrix Metalloproteinases) interact with SPARCL1 affecting tissue integrity and repair. In the focal adhesion pathway SPARCL1 affects cell shape and movement by regulating interactions with integrins which are integral membrane proteins that communicate the extracellular matrix to the cell.
- Associated diseases and disorders
SPARCL1 has associations with cancer and neuropathological diseases. Reduced expression of SPARCL1 often occurs in various cancers including prostate and colorectal cancers suggesting its potential role as a tumor suppressor. In neuropathological conditions such as Alzheimer's disease altered SPARCL1 expression affects neuroinflammation and synaptic functions related to the amyloid precursor protein (APP) which plays a critical role in Alzheimer's pathology. Understanding these pathways offers insight into therapeutic potential in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunocytochemistry-immunofluorescence-img418491.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Immunocytochemistry/ Immunofluorescence - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling SPARCL1 with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/50 (9.9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse astrocyte cells.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.![Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunoprecipitation-img418428.jpg "Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
SPARCL1 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Anti-SPARCL1 antibody [EPR28135-54] ab313638 IP in Mouse cerebellum tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SPARCL1 antibody [EPR28135-54] ab313638 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 secondsAll lanes: Immunoprecipitation - Anti-SPARCL1 antibody [EPR28135-54] (Anti-SPARCL1 antibody [EPR28135-54] ab313638) at 1/30 dilution
All lanes: Mouse cerebellum tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Exposure time: 10s
SPARCL1 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Anti-SPARCL1 antibody [EPR28135-54] ab313638 IP in Mouse cerebellum tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SPARCL1 antibody [EPR28135-54] ab313638 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds![Western blot - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--western-blot-img418414.jpg "Western blot - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Western blot - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 22173850).
Low expression: kidney (PMID: 9199668; PMID: 32437418).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 6 secondsAll lanes: Western blot - Anti-SPARCL1 antibody [EPR28135-54] (Anti-SPARCL1 antibody [EPR28135-54] ab313638) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse hippocampus tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 130 kDa
Exposure time: 6s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 22173850).
Low expression: kidney (PMID: 9199668; PMID: 32437418).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 6 seconds![Flow Cytometry (Intracellular) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--flow-cytometry-intracellular-img418505.jpg "Flow Cytometry (Intracellular) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Flow Cytometry (Intracellular) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse primary neural/glia cell cells labelling SPARCL1 with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418473.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling SPARCL1 with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/2000 (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on endothelium of mouse spleen (PMID: 9199668).The section was incubated with Anti-SPARCL1 antibody [EPR28135-54] ab313638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418472.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)
This data was developed using Anti-SPARCL1 antibody [EPR28135-54] ab313638, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SPARCL1 with Anti-SPARCL1 antibody [EPR28135-54] ab313638 at 1/2000 (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 18381651).The section was incubated with Anti-SPARCL1 antibody [EPR28135-54] ab313638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639), expandable thumbnail](https://content.abcam.com/products/images/sparcl1-antibody-epr28135-54-bsa-and-azide-free-ab313639--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418472.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR28135-54] - BSA and Azide free (ab313639)")
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