JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB167190

Anti-Spartan antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Polyclonal Spartan antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPRTN.

View Alternative Names

C1orf124, DVC1, UNQ1880/PRO4323, SPRTN, DNA-dependent metalloprotease SPRTN, DNA damage protein targeting VCP, Protein with SprT-like domain at the N terminus, Spartan

1 Images
Western blot - Anti-Spartan antibody (AB167190)
  • WB

Unknown

Western blot - Anti-Spartan antibody (AB167190)

All lanes:

Western blot - Anti-Spartan antibody (ab167190) at 1 µg/mL

Lane 1:

Lysate of 293T cells transfected with Spartan at 15 µL

Lane 2:

Lysate of 293T cells (non-transfected) at 15 µL

Predicted band size: 103 kDa,40 kDa,55 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SPRTN.

Q9H040

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spartan also known as SPRTN (DNA Sequence Nonspecific) is a metalloprotease enzyme weighing approximately 53 kDa. It is an important player in genome maintenance and DNA repair especially in the stress response to DNA replication impediments. SPRTN is widely expressed in various tissues with particularly high expression in proliferative tissues where DNA replication is important such as the liver and bone marrow. Its main mechanical role involves recognizing and processing DNA-protein crosslinks that may hinder replication and transcription processes.
Biological function summary

SPRTN is important for DNA replication fidelity and cellular response to DNA damage. It achieves this by being part of the DNA-protein crosslink repair pathway where it cooperates with other proteins to maintain genomic stability. This function ensures replication continues correctly and prevents harmful mutations. SPRTN operates as a protease to cleave proteins trapped on DNA thereby removing obstacles and allowing replication machinery to advance. This activity demonstrates its vital role in safeguarding genetic information.

Pathways

SPRTN integrates into the DNA damage response pathways such as the Fanconi Anemia pathway coordinating with other proteins to maintain genomic integrity. Additionally SPRTN associates with the homologous recombination repair pathway where it interacts with proteins like RAD51 and BRCA1 to facilitate effective DNA repair. These pathways are essential for managing DNA damage events to minimize the risk of genomic instabilities that may lead to disorders.

SPRTN mutations have strong links to a rare genetic disorder known as Ruijs-Aalfs Syndrome characterized by progeroid features and cancer predisposition due to accumulated DNA damage. This connection highlights its role in maintaining genomic stability. Moreover deficiency in SPRTN has relevance to cancer development as its impaired function leads to compromised DNA repair allowing mutations to accumulate. The loss of this protease's activity can correlate with destabilization of genomic maintenance partners like FANCD2 potentially contributing to oncogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 30893605, PubMed : 32649882, PubMed : 36608669). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 32649882, PubMed : 36608669). Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 32649882). Catalyzes proteolytic cleavage of the HMCES DNA-protein cross-link following unfolding by the BRIP1/FANCJ helicase (PubMed : 36608669). Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4 (PubMed : 27871366). Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs (By similarity). SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs (By similarity). Involved in recruitment of VCP/p97 to sites of DNA damage (PubMed : 22902628, PubMed : 23042605, PubMed : 23042607, PubMed : 32152270). Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation (PubMed : 31316063). Does not activate CHEK1 in response to DNA damage (PubMed : 31316063). May also act as a 'reader' of ubiquitinated PCNA : recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA (PubMed : 22681887, PubMed : 22894931, PubMed : 22902628, PubMed : 22987070). Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis (PubMed : 22681887).
See full target information SPRTN
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB121853

Anti-Spartan antibody

Immunocytochemistry/ Immunofluorescence - Anti-Spartan antibody (AB121853)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB124711

Anti-ATG10 antibody [EPR4804]

RabMAb|Recombinant|KO Validated

Western blot - Anti-ATG10 antibody [EPR4804] (AB124711)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Cell Lines & Lysates

AB265682

Human ATG10 knockout HeLa cell line

Advanced Validation

Western blot - Human ATG10 knockout HeLa cell line (AB265682)

  • 0
  • 0
Primary Antibodies

AB168643

Anti-PRL-1 antibody

Western blot - Anti-PRL-1 antibody (AB168643)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com