AB180154

Anti-Spata18 antibody [EPR13704]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Spata18 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

MIEAP, SPATA18, Mitochondria-eating protein, Spermatogenesis-associated protein 18

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (AB180154)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (AB180154)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (AB180154)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (AB180154)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Spata18 antibody [EPR13704] (AB180154)

All lanes:

Western blot - Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

LnCaP cell lysate at 20 µg

Lane 4:

HT1080 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13704

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spata18 also known as MitoPLD or spermatogenesis-associated protein 18 is an important protein that participates in mitochondrial quality control. This protein has a molecular mass of approximately 74 kDa. Researchers find Spata18 expressed mainly in the testes and other tissues such as heart and skeletal muscle. Spata18 acts on the mitochondria by mediating mitophagy which is the selective degradation of damaged or dysfunctional mitochondria thereby maintaining healthy cellular function.

Biological function summary

Spata18 exerts significant roles that ensure proper cell functionality. It initiates mitophagy by facilitating the mitochondria's removal when they become damaged preventing the accumulation of mitochondrial reactive oxygen species (ROS). Spata18 is known to function independently and does not typically require a larger protein complex to mediate its activity. Its expression and activity aggressively safeguard cellular integrity against oxidative stress by assuring the removal of compromised mitochondria.

Pathways

Spata18 is involved in the mitochondrial quality control and autophagy pathways. This protein significantly affects the pathways closely related to mitochondrial dynamics and function specifically promoting autophagic processes and mitochondria turnover. In association with other proteins like PINK1 and Parkin Spata18 operates within the mitophagy pathway to oversee the clearance of damaged mitochondria maintaining cellular health and bioenergetics balance.

Spata18's involvement in mitochondrial health can influence the onset and progression of conditions like Parkinson's disease and cardiac disorders. The protein's effective role in promoting mitophagy becomes important in neurodegenerative diseases as improper mitophagy and mitochondrial dysfunction appear as common features. In the context of Parkinson's disease Spata18 associates with Parkin and helps mitigate the effects of mitochondrial damage. Additionally its expression in cardiac tissues hints at potential links to cardiac disorders although further research is necessary to concrete its clinical significance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.

See full target information SPATA18

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7316 PubMed40781068

2025

Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Laurel E Kelnhofer-Millevolte,Julian R Smith,Daniel H Nguyen,Lea S Wilson,Hannah C Lewis,Edward A Arnold,Mia R Brinkley,Kihye Shin,Jin-Hyun Ahn,Eui Tae Kim,Katarzyna Kulej,Adam P Geballe,Srinivas Ramachandran,Daphne C Avgousti

Frontiers in endocrinology 13:932754 PubMed36187114

2022

MIEAP and ATG5 are tumor suppressors in a mouse model of BRAF-positive thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Koichiro Hamada,Tomomi Kurashige,Mika Shimamura,Hirofumi Arakawa,Yasuyuki Nakamura,Yuji Nagayama

Antioxidants (Basel, Switzerland) 11: PubMed35883794

2022

Impact of Roux-en-Y Gastric Bypass on Mitochondrial Biogenesis and Dynamics in Leukocytes of Obese Women.

Applications

Unspecified application

Species

Unspecified reactive species

Zaida Abad-Jiménez,Teresa Vezza,Sandra López-Domènech,Meylin Fernández-Reyes,Francisco Canet,Carlos Morillas,Segundo Ángel Gómez-Abril,Celia Bañuls,Víctor M Víctor,Milagros Rocha

Cells 10: PubMed34572103

2021

Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Won-Hee Song,Dalen Zuidema,Young-Joo Yi,Michal Zigo,Zhibing Zhang,Miriam Sutovsky,Peter Sutovsky

The Journal of cell biology 218:1491-1502 PubMed30914420

2019

Extramitochondrial cardiolipin suggests a novel function of mitochondria in spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mindong Ren,Yang Xu,Hediye Erdjument-Bromage,Alec Donelian,Colin K L Phoon,Naohiro Terada,Douglas Strathdee,Thomas A Neubert,Michael Schlame

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spata18 antibody [EPR13704]

Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (AB180154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (AB180154)

Western blot - Anti-Spata18 antibody [EPR13704] (AB180154)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise