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Rabbit Recombinant Monoclonal SPC24 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (AB249292), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (AB249292), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).

Alternative names

SPBC24, SPC24, Kinetochore protein Spc24, hSpc24

Recommended products

Rabbit Recombinant Monoclonal SPC24 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11547(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249292 is the carrier-free version of Anti-SPC24 antibody [EPR11547(B)] ab157184.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SPC24 protein also known as Spindle Pole Body Component 24 weighs around 22 kDa. SPC24 is expressed in various tissues but it shows higher levels in dividing cells particularly during mitosis. Mechanically it forms an essential part of the NDC80 complex contributing to kinetochore-microtubule connections that are important for chromosome segregation during cell division. SPC24 interacts with microtubules through the NDC80 complex ensuring accurate chromosome alignment and stabilization.

Biological function summary

SPC24 plays a role in maintaining genomic stability and its function is highly dependent on the NDC80 complex. This complex is an integral component of the kinetochore a structure on chromosomes critical for equal distribution during cell division. By anchoring microtubules to kinetochores SPC24 helps prevent aneuploidy which can arise from improper chromosomal segregation. The presence of SPC24 supports robust kinetochore-microtubule attachments which are important for precise mitotic progression and cellular proliferation.

Pathways

SPC24 is involved in the cell cycle especially during mitosis. In the mitotic pathway it assists in the regulation of spindle checkpoint signaling ensuring each daughter cell receives the correct number of chromosomes. This ensures proper mitotic checkpoint control. SPC24 interfaces with other proteins such as SPC25 within the NDC80 complex and interacts with signaling pathways involving kinases like Aurora B which monitors and corrects kinetochore-microtubule attachments.

Associated diseases and disorders

SPC24 plays a role in cancer progression notably in cases where chromosomal instability is high such as in colorectal cancer. Aberrations in SPC24 function or expression can lead to improper kinetochore tension and chromosomal missegregation worsening this instability. SPC24 can relate to the enhancement of aggressive cancer phenotypes acting in conjunction with proteins like Mis12 which also contributes to kinetochore function. Targeting SPC24 could offer pathways for therapeutic strategies in treating cancers marked by mitotic errors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292), expandable thumbnail

    Western blot - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292)

    This data was developed using Anti-SPC24 antibody [EPR11547(B)] ab157184, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: MOLT4 cell lysate at 10 µg

    Lane 3: TF1 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: Human thymus tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292)

    This data was developed using Anti-SPC24 antibody [EPR11547(B)] ab157184, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells, labeling SPC24 with Anti-SPC24 antibody [EPR11547(B)] ab157184 at 1/50 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com