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AB236972

Anti-SPCS2/SPC25 antibody

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(2 Publications)

Rabbit Polyclonal SPCS2/SPC25 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPCS2 aa 1-100.

View Alternative Names

KIAA0102, SPC25, SPCS2, Signal peptidase complex subunit 2, Microsomal signal peptidase 25 kDa subunit, SPase 25 kDa subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPCS2/SPC25 antibody (AB236972)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPCS2/SPC25 antibody (AB236972)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SPCS2/SPC25 (green) using ab236972 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-SPCS2/SPC25 antibody (AB236972)
  • WB

Supplier Data

Western blot - Anti-SPCS2/SPC25 antibody (AB236972)

All lanes:

Western blot - Anti-SPCS2/SPC25 antibody (ab236972) at 1/500 dilution

All lanes:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB236972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB236972)

Paraffin-embedded human colon cancer tissue stained for SPCS2/SPC25 using ab236972 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB236972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS2/SPC25 antibody (AB236972)

Paraffin-embedded human testis tissue stained for SPCS2/SPC25 using ab236972 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPCS2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q15005

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPCS2 also called SPC25 is a component of the signal peptidase complex involved in cleaving signal peptides from nascent proteins during translocation into the endoplasmic reticulum. This protein has a molecular mass of approximately 27 kDa. SPCS2 is expressed in various tissues with notable levels in the liver pancreas and kidney. This broad expression suggests its importance across multiple physiological systems.
Biological function summary

The function of SPCS2/SPC25 relates to its role in the signal peptidase complex. It participates in the maturation of proteins by processing them for proper folding and export within the secretory pathway. As part of this multimeric complex in the endoplasmic reticulum membrane SPCS2 coordinates with other components like SEC11 to efficiently cleave signal peptides. Its function is critical for the regulation of protein synthesis and secretion processes.

Pathways

SPCS2 functions within the secretory pathways particularly in protein translocation into the endoplasmic reticulum. Its involvement in the ER-associated degradation pathway highlights its role in maintaining protein homeostasis. Related proteins such as SEC61 and ERAB are key associates in these pathways working together to facilitate the translocation and processing of nascent proteins entering the ER.

SPCS2 is linked with pancreatic diseases and certain liver dysfunctions due to its role in protein processing and secretion. Aberrant functioning of SPCS2 can disrupt normal protein folding and maturation potentially leading to conditions like pancreatitis. The protein's link to SEC11 in the protein processing pathways highlights shared dysfunction pathways that can influence disease severity and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 34388369). Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site (By similarity).
See full target information SPCS2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:780 PubMed35918402

2022

Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rossella Delle Donne,Rosa Iannucci,Laura Rinaldi,Luca Roberto,Maria A Oliva,Emanuela Senatore,Domenica Borzacchiello,Luca Lignitto,Giorgio Giurato,Francesca Rizzo,Assunta Sellitto,Francesco Chiuso,Salvatore Castaldo,Giovanni Scala,Virginia Campani,Valeria Nele,Giuseppe De Rosa,Chiara D'Ambrosio,Corrado Garbi,Andrea Scaloni,Alessandro Weisz,Concetta Ambrosino,Antonella Arcella,Antonio Feliciello

Frontiers in immunology 13:907636 PubMed35967419

2022

Spindle pole body component 25 and platelet-derived growth factor mediate crosstalk between tumor-associated macrophages and prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feilun Cui,Zhipeng Xu,Jianpeng Hu,Yumei Lv
View all publications
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