AB242101

Anti-SPCS2/SPC25 antibody

Name: Anti-SPCS2/SPC25 antibody (ab242101) | Abcam
Brand: Abcam Limited
SKU: AB242101
Price: 520 USD
Availability: InStock

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Rabbit Polyclonal SPCS2/SPC25 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SPCS2 aa 150 to C-terminus.

View Alternative Names

KIAA0102, SPC25, SPCS2, Signal peptidase complex subunit 2, Microsomal signal peptidase 25 kDa subunit, SPase 25 kDa subunit

2 Images

Supplier Data

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (AB242101)

SPCS2/SPC25 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242101 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab242101 at 1/250 dilution.

Lane 1 : ab242101 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (ab242101)

Predicted band size: 25 kDa

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Supplier Data

Western blot - Anti-SPCS2/SPC25 antibody (AB242101)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-SPCS2/SPC25 antibody (ab242101) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 25 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SPCS2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15005

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to SPCS2/SPC25 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPCS2 also called SPC25 is a component of the signal peptidase complex involved in cleaving signal peptides from nascent proteins during translocation into the endoplasmic reticulum. This protein has a molecular mass of approximately 27 kDa. SPCS2 is expressed in various tissues with notable levels in the liver pancreas and kidney. This broad expression suggests its importance across multiple physiological systems.

Biological function summary

The function of SPCS2/SPC25 relates to its role in the signal peptidase complex. It participates in the maturation of proteins by processing them for proper folding and export within the secretory pathway. As part of this multimeric complex in the endoplasmic reticulum membrane SPCS2 coordinates with other components like SEC11 to efficiently cleave signal peptides. Its function is critical for the regulation of protein synthesis and secretion processes.

Pathways

SPCS2 functions within the secretory pathways particularly in protein translocation into the endoplasmic reticulum. Its involvement in the ER-associated degradation pathway highlights its role in maintaining protein homeostasis. Related proteins such as SEC61 and ERAB are key associates in these pathways working together to facilitate the translocation and processing of nascent proteins entering the ER.

SPCS2 is linked with pancreatic diseases and certain liver dysfunctions due to its role in protein processing and secretion. Aberrant functioning of SPCS2 can disrupt normal protein folding and maturation potentially leading to conditions like pancreatitis. The protein's link to SEC11 in the protein processing pathways highlights shared dysfunction pathways that can influence disease severity and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 34388369). Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site (By similarity).

See full target information SPCS2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPCS2/SPC25 antibody

Immunoprecipitation - Anti-SPCS2/SPC25 antibody (AB242101)

Western blot - Anti-SPCS2/SPC25 antibody (AB242101)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise