AB235598

Anti-SPCS3 antibody

Name: Anti-SPCS3 antibody (ab235598) | Abcam
Brand: Abcam Limited
SKU: AB235598
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SPCS3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPCS3 aa 1 to C-terminus.

View Alternative Names

SPC22, UNQ1841/PRO3567, SPCS3, Signal peptidase complex subunit 3, Microsomal signal peptidase 22/23 kDa subunit, SPC22/23, SPase 22/23 kDa subunit

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemical analysis of ab235598 diluted at 1 : 150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPCS3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61009

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPCS3 also known as Signal Peptidase Complex Subunit 3 functions mechanically as a component of the signal peptidase complex which is responsible for cleaving the signal peptides from nascent proteins. It plays an important role in the protein maturation process within the endoplasmic reticulum. The protein is approximately 15 kDa in mass and is highly expressed in tissues such as the liver and pancreas. Its expression is critical in cells where active protein synthesis occurs.

Biological function summary

The signal peptidase complex including SPCS3 is involved in the processing and maturation of secretory and membrane proteins. SPCS3 forms part of a larger multisubunit complex that ensures proteins assume their proper functional forms. By cleaving signal peptides SPCS3 facilitates the proper localization and function of proteins within the cell. In doing so it contributes to cellular processes that depend on effective signal peptide processing such as growth and development.

Pathways

SPCS3 plays a significant role in the protein processing pathway in the endoplasmic reticulum. It interacts with other proteins involved in this pathway such as SPCS1 and SEC61 ensuring the correct translocation and maturation of newly synthesized polypeptides. The efficient functioning of SPCS3 in these pathways is important for maintaining cellular homeostasis and supporting protein synthesis and secretion.

Research links SPCS3 to conditions involving protein misfolding and secretion dysfunction such as cystic fibrosis and some forms of diabetes. In cystic fibrosis the malfunction of SPCS3 can lead to improper processing of the CFTR protein exacerbating disease symptoms. The protein's role in these diseases connects it to other factors involved in the pathology such as the CFTR protein. Understanding how SPCS3 influences these disorders can help develop targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed : 27499293, PubMed : 34388369). Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface (By similarity).. (Microbial infection) Plays an important role in virion production of flaviviruses such as West Nile virus, Japanese enchephalitis virus, Dengue virus type 2 and Yellow Fever virus.

See full target information SPCS3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 24:681 PubMed38834966

2024

PPIH acts as a potential predictive biomarker for patients with common solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ye,Jianchao Ying,Haixia Chen,Zhiping Wu,Chaolin Huang,Chuan Zhang,Zhitao Chen,Haini Chen

Nucleic acids research 52:4872-4888 PubMed38412296

2024

RNA-RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Ressel,Sujai Kumar,Jose Roberto Bermúdez-Barrientos,Katrina Gordon,Julia Lane,Jin Wu,Cei Abreu-Goodger,Jürgen Schwarze,Amy H Buck

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPCS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise