Rabbit Polyclonal SPDEF/PSE antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPDEF aa 50-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SPDEF aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O95238
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
Alternative names
PDEF, PSE, SPDEF, SAM pointed domain-containing Ets transcription factor, Prostate epithelium-specific Ets transcription factor, Prostate-derived Ets factor, Prostate-specific Ets
Recommended products
Rabbit Polyclonal SPDEF/PSE antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPDEF aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SPDEF aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O95238
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPDEF also known as SAM pointed domain-containing ETS transcription factor is a protein with a mass of approximately 42 kDa. It functions as a transcription factor primarily regulating the expression of genes involved in cellular differentiation and proliferation. SPDEF is expressed mainly in epithelial tissues notably in the prostate lung and gastrointestinal tract. This expression pattern highlights its role in maintaining proper tissue homeostasis and function.
- Biological function summary
SPDEF facilitates the differentiation of epithelial cells by influencing the transcription of lineage-specific genes. It interacts with other transcription factors and co-factors forming complexes to modulate gene expression with precision. Its involvement in mucin production defines a protective mechanism in epithelial lining contributing to barrier integrity. SPDEF's role in differentiation makes it an important player in epithelial tissue maintenance and function.
- Pathways
SPDEF participates actively in the Wnt signaling pathway and the PI3K/AKT pathway. In the Wnt pathway it modulates the gene expression program to influence cell fate decisions often in collaboration with β-catenin. Regarding the PI3K/AKT pathway its role impacts cell survival and growth. SPDEF's interactions with proteins like FOXA1 and AR (Androgen Receptor) through these pathways draw a complex map of gene regulatory networks essential for cell function.
- Associated diseases and disorders
SPDEF is linked to prostate cancer and asthma. In prostate cancer aberrant SPDEF activity or expression influences tumor progression and metastasis often interacting with proteins such as AR. Conversely its role in asthma involves the regulation of mucin genes making it significant in airway hyperresponsiveness and remodeling. The connection between SPDEF and these diseases illuminates the overarching role of this transcription factor in health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunocytochemistry/ Immunofluorescence - Anti-SPDEF/PSE antibody (ab220776)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling SPDEF/PSE with ab220776 at 4 μg/ml (green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com