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AB220776

Anti-SPDEF/PSE antibody

1

(1 Review)

|

(2 Publications)

Rabbit Polyclonal SPDEF/PSE antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPDEF aa 50-200.

View Alternative Names

PDEF, PSE, SPDEF, SAM pointed domain-containing Ets transcription factor, Prostate epithelium-specific Ets transcription factor, Prostate-derived Ets factor, Prostate-specific Ets

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPDEF/PSE antibody (AB220776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPDEF/PSE antibody (AB220776)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling SPDEF/PSE with ab220776 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SPDEF aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O95238

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Product details

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPDEF also known as SAM pointed domain-containing ETS transcription factor is a protein with a mass of approximately 42 kDa. It functions as a transcription factor primarily regulating the expression of genes involved in cellular differentiation and proliferation. SPDEF is expressed mainly in epithelial tissues notably in the prostate lung and gastrointestinal tract. This expression pattern highlights its role in maintaining proper tissue homeostasis and function.
Biological function summary

SPDEF facilitates the differentiation of epithelial cells by influencing the transcription of lineage-specific genes. It interacts with other transcription factors and co-factors forming complexes to modulate gene expression with precision. Its involvement in mucin production defines a protective mechanism in epithelial lining contributing to barrier integrity. SPDEF's role in differentiation makes it an important player in epithelial tissue maintenance and function.

Pathways

SPDEF participates actively in the Wnt signaling pathway and the PI3K/AKT pathway. In the Wnt pathway it modulates the gene expression program to influence cell fate decisions often in collaboration with β-catenin. Regarding the PI3K/AKT pathway its role impacts cell survival and growth. SPDEF's interactions with proteins like FOXA1 and AR (Androgen Receptor) through these pathways draw a complex map of gene regulatory networks essential for cell function.

SPDEF is linked to prostate cancer and asthma. In prostate cancer aberrant SPDEF activity or expression influences tumor progression and metastasis often interacting with proteins such as AR. Conversely its role in asthma involves the regulation of mucin genes making it significant in airway hyperresponsiveness and remodeling. The connection between SPDEF and these diseases illuminates the overarching role of this transcription factor in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
See full target information SPDEF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2764 PubMed38553466

2024

Identification of spatially-resolved markers of malignant transformation in Intraductal Papillary Mucinous Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Agostini,Geny Piro,Frediano Inzani,Giuseppe Quero,Annachiara Esposito,Alessia Caggiano,Lorenzo Priori,Alberto Larghi,Sergio Alfieri,Raffaella Casolino,Giulia Scaglione,Vincenzo Tondolo,Giovanni Cammarota,Gianluca Ianiro,Vincenzo Corbo,Andrew V Biankin,Giampaolo Tortora,Carmine Carbone

Genes 12: PubMed34356103

2021

Heterozygous Mutation Affects Glucose Tolerance in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Šedová,Jan Prochazka,Dagmar Zudová,Běla Bendlová,Josef Včelák,Radislav Sedlacek,Ondřej Šeda
View all publications
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Product promise

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