Anti-Spectrin beta-3 antibody [RP23040210]

• WB

Supplier Data

Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (AB308134)

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), HCT 116 (Lane 3), Caco-2 (Lane 4), MCF7 (Lane 5), T47D (Lane 6), THP-1 (Lane 7) and KARPAS 299 (Lane 8). The blots were probed with Anti-Spectrin Beta 3 Recombinant Rabbit Polyclonal Antibody (ab308134, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 268 kDa band corresponding to Spectrin Beta 3 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane with an overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (ab308134) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HT-29 whole cell lysate at 30 µg

Lane 3:

HCT 116 whole cell lysate at 30 µg

Lane 4:

Caco-2 whole cell lysate at 30 µg

Lane 5:

MCF7 whole cell lysate at 30 µg

Lane 6:

T47D whole cell lysate at 30 µg

Lane 7:

THP-1 whole cell lysate at 30 µg

Lane 8:

KARPAS 299 whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 271 kDa

Observed band size: 268 kDa

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