Rabbit Multiclonal SPTBN2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPTBN2.
Images
![Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (AB308134), expandable thumbnail](https://content.abcam.com/products/images/spectrin-beta-3-antibody-rp23040210-ab308134--western-blot-img406962.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (AB308134)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
Liquid
- Clonality
Multiclonal
Immunogen
- Synthetic Peptide within Human SPTBN2. Database link O15020
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Target data
Function
Probably plays an important role in neuronal membrane skeleton.
Alternative names
Beta-III spectrin, Spinocerebellar ataxia 5 protein, SPTBN2, KIAA0302, SCA5
Recommended products
Rabbit Multiclonal SPTBN2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPTBN2.
Alternative names
Beta-III spectrin, Spinocerebellar ataxia 5 protein, SPTBN2, KIAA0302, SCA5
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
Liquid
- Clonality
Multiclonal
- Immunogen
- Synthetic Peptide within Human SPTBN2. Database link O15020
- Clone number
RP23040210
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (ab308134), expandable thumbnail](https://content.abcam.com/products/images/spectrin-beta-3-antibody-rp23040210-ab308134--western-blot-img406962.jpg "Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (ab308134)")
Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (ab308134)
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), HCT 116 (Lane 3), Caco-2 (Lane 4), MCF7 (Lane 5), T47D (Lane 6), THP-1 (Lane 7) and KARPAS 299 (Lane 8). The blots were probed with Anti-Spectrin Beta 3 Recombinant Rabbit Polyclonal Antibody (ab308134, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500 dilution). A 268 kDa band corresponding to Spectrin Beta 3 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane with an overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).
All lanes: Western blot - Anti-Spectrin beta-3 antibody [RP23040210] (ab308134) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HT-29 whole cell lysate at 30 µg
Lane 3: HCT 116 whole cell lysate at 30 µg
Lane 4: Caco-2 whole cell lysate at 30 µg
Lane 5: MCF7 whole cell lysate at 30 µg
Lane 6: T47D whole cell lysate at 30 µg
Lane 7: THP-1 whole cell lysate at 30 µg
Lane 8: KARPAS 299 whole cell lysate at 30 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 271 kDa
Observed band size: 268 kDa
Downloads
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