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AB241997

Anti-Spectrin beta chain, brain 3 antibody [S393-2]

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(1 Publication)

Mouse Monoclonal Spectrin beta chain, brain 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPTBN4 aa 1600-1850.

View Alternative Names

KIAA1642, SPTBN3, SPTBN4, Beta-IV spectrin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)

4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Spectrin beta chain, brain 3 (green, C) using ab241997 at 1/100 dilution for 60 minutes at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.

Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Spectrin beta chain with ab241997 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) beta 4 Spectrin Antibody (D) Composite.

Western blot - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)
  • WB

Supplier Data

Western blot - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (AB241997)

Primary incubation for 16 hrs at 4°C.

Secondary incubation for 1 hr at RT.

Blocked for 1 hr at RT.

All lanes:

Western blot - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997) at 1/1000 dilution

All lanes:

COS cells over-expressing human Spectrin beta chain, brain 3-His protein at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/200 dilution

Predicted band size: 289 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S393-2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPTBN4 aa 1600-1850. The exact immunogen used to generate this antibody is proprietary information.

Q9H254

Specificity

Does not cross react with other Beta-spectrins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spectrin beta chain brain 3 also known as SPTBN3 is a cytoskeletal protein that belongs to the spectrin family. It displays a mass of approximately 271 kDa. This protein is ubiquitously expressed in the brain where it contributes to the cytoskeletal framework by cross-linking actin filaments. Additionally it plays a structural role in maintaining the integrity and flexibility of cell membranes. SPTBN3 is essential for proper neuronal function by stabilizing membrane proteins and supporting intracellular transport.
Biological function summary

Spectrin beta chain brain 3 functions as part of the spectrin cytoskeleton which involves binding with alpha spectrin to form a heterodimer further assembling into a tetramer structure. This spectrin network supports the cell's structural organization and is critical in processes like signal transduction and protein targeting. Its presence in neurons aids in maintaining the shape and polarity required for the proper functioning of complex neural networks. The spectrin-based cytoskeleton interacts with numerous other proteins to modulate various cellular activities.

Pathways

SPTBN3 holds relevance in the regulation of the MAPK signaling pathway and the actin cytoskeleton pathway. The MAPK pathway is important for transmitting extracellular signals to responses like cell growth and differentiation. As a result SPTBN3 interacts with proteins such as MAP2K1 facilitating proper signal propagation. In the actin cytoskeleton pathway SPTBN3 maintains cellular morphology and mechanical support working with proteins like actin beta contributing to various morphological processes.

Spectrin beta chain brain 3 connects to neurodegenerative disorders and epilepsy. Abnormalities in SPTBN3 expression or function may result in neuronal instability leading to conditions like Alzheimer's disease due to disrupted cytoskeletal dynamics. Changes in its interaction with proteins such as tau and amyloid precursor protein can exacerbate pathological processes. Its role in epilepsy emerges through dysfunctional synaptic stability and neuronal signaling emphasizing SPTBN3's importance in maintaining normal neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SPTBN4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 12:e1194 PubMed38501544

2024

Sodium butyrate activates the K channels to regulate the mechanism of Parkinson's disease microglia model inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Xu,Laofu Wen,Yunyi Tang,Zhenqiang Zhao,Miaojing Xu,Tan Wang,Zhibin Chen
View all publications
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