Rabbit Polyclonal SPEF1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPEF1 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SPEF1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4P9
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
Target data
Function
Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (By similarity). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (PubMed:31473225). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity).
Alternative names
C20orf28, SPEF1, Sperm flagellar protein 1
Recommended products
Rabbit Polyclonal SPEF1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPEF1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SPEF1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4P9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The antibody solution should be gently mixed before use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPEF1 also known as Sperm Flagellar Protein 1 functions in the motility of cilia and flagella. The protein has a mass of approximately 38 kDa. Researchers have observed its expression mainly in tissues with motile cilia such as the respiratory tract and reproductive system. It is essential for proper ciliary movement contributing to the physical structure and movement capability of cilia and flagella.
- Biological function summary
As part of motility mechanisms SPEF1 plays a role in the assembly and stabilization of the axoneme the core structure of cilia and flagella. This protein is an axonemal protein that interacts with microtubules facilitating the sliding of microtubule doublets necessary for ciliary beating. SPEF1 does not act alone; it works within a multi-protein complex that includes other ciliary proteins to ensure effective ciliary and flagellar function.
- Pathways
SPEF1 participates in pathways involved in respiratory cilia function and sperm motility. Within these pathways its role is connected to proteins such as dynein and kinesin which are also involved in microtubule-based movements. These pathways are essential for proper respiratory function and fertility due to their involvement in the movement and fluid clearance by cilia as well as sperm motility.
- Associated diseases and disorders
SPEF1 dysregulation links to primary ciliary dyskinesia (PCD) and some infertility cases. PCD results from mutated components of the axoneme impacting SPEF1 along with other proteins like DNAI1. These conditions illustrate the importance of functional ciliary and flagellar proteins for respiratory health and reproduction. Understanding the exact role of SPEF1 in these disorders helps in the development of targeted therapies and diagnostic tools.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEF1 antibody (ab151100)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SPEF1 with ab151100 at 1/50 dilution.
Western blot - Anti-SPEF1 antibody (ab151100)
All lanes: Western blot - Anti-SPEF1 antibody (ab151100) at 1/250 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human Plasma lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Predicted band size: 27 kDa
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