AB72266

Anti-SPEN antibody

Name: Anti-SPEN antibody (ab72266) | Abcam
Brand: Abcam Limited
SKU: AB72266
Price: 515 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal SPEN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SPEN.

View Alternative Names

KIAA0929, MINT, SHARP, SPEN, Msx2-interacting protein, SMART/HDAC1-associated repressor protein, SPEN homolog

1 Images

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Immunoprecipitation - Anti-SPEN antibody (AB72266)

Immunoprecipitation/ Western Blot of SPEN.
Lane 1 : ab72266 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab72266 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-SPEN antibody (ab72266)

Predicted band size: 402 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SPEN. The exact immunogen used to generate this antibody is proprietary information.

Q96T58

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPEN also known as Split Ends or SHARP is a large nuclear protein with a mass of approximately 400 kDa. It is expressed in various tissues including the brain heart and lungs. Mechanically SPEN functions as an RNA-binding protein with C-terminal SPOC (Spen Paralog and Ortholog C-terminal) domains which allow it to interact with other cellular components. These interactions regulate transcription by modulating the activity of coactivators and corepressors at specific gene sites.

Biological function summary

SPEN plays a role in transcriptional repression and gene silencing by being part of a multiprotein complex. It interacts with nuclear receptors and RNA polymerase II inhibiting the transcriptional activity of target genes. SPEN helps regulate critical cellular processes such as differentiation and proliferation. It acts as a transcriptional corepressor by interacting with nucleic acids and influencing chromatin structure impacting gene expression in a context-dependent manner.

Pathways

SPEN holds an important role in the Notch signaling pathway. This pathway is important for cell fate determination and tissue homeostasis. SPEN also interacts with the estrogen receptor signaling pathway where it can influence the transcriptional outcome. Through these pathways SPEN associates with proteins such as RBPJ a transcriptional regulator in the Notch pathway and ERα (estrogen receptor alpha) a vital component in the estrogen signaling pathway.

SPEN has connections to certain cancers particularly breast cancer and neurological conditions like autism spectrum disorders. In breast cancer SPEN can interact with ERα influencing the proliferation of cancer cells through estrogen receptor signaling. In neurological conditions dysregulation of SPEN's interaction with proteins involved in neural development might contribute to disease mechanisms. Understanding these associations helps in potential therapeutic targeting of SPEN in relevant diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation : synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.

See full target information SPEN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1583752 PubMed40391156

2025

Rewired glycolysis by DTL accelerates oncometabolite L-lactate generation to promote breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhao Liu,Jinting Li,Yiren Cao,Mengzhu Lv

Nature structural & molecular biology 31:1589-1600 PubMed38834912

2024

XIST dampens X chromosome activity in a SPEN-dependent manner during early human development.

Applications

Unspecified application

Species

Unspecified reactive species

Charbel Alfeghaly,Gaël Castel,Emmanuel Cazottes,Madeleine Moscatelli,Eva Moinard,Miguel Casanova,Juliette Boni,Kasturi Mahadik,Jenna Lammers,Thomas Freour,Louis Chauviere,Carla Piqueras,Ruben Boers,Joachim Boers,Joost Gribnau,Laurent David,Jean-François Ouimette,Claire Rougeulle

BMC cancer 23:793 PubMed37620924

2023

Pan-cancer analysis identifies SPEN mutation as a predictive biomarker with the efficacy of immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Dong Li,Hao Huang,Zheng-Ju Ren,Ye Yuan,Hao Wu,Chuan Liu

Nature communications 12:7000 PubMed34853312

2021

SPEN is required for Xist upregulation during initiation of X chromosome inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Robert-Finestra,Beatrice F Tan,Hegias Mira-Bontenbal,Erika Timmers,Cristina Gontan,Sarra Merzouk,Benedetto Daniele Giaimo,François Dossin,Wilfred F J van IJcken,John W M Martens,Tilman Borggrefe,Edith Heard,Joost Gribnau

Cell death & disease 11:509 PubMed32641685

2020

SPEN induces miR-4652-3p to target HIPK2 in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Yumin Lv,Chao Cheng,Yan Huang,Liu Yang,Jingjing He,Xingyu Tao,Yingying Hu,Yuting Ma,Yun Su,Liyang Wu,Guifang Yu,Qingping Jiang,Shu Liu,Xiong Liu,Zhen Liu

Reproduction (Cambridge, England) 156:195-206 PubMed29880719

2018

SPEN protein expression and interactions with chromatin in mouse testicular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Korfanty,Tomasz Stokowy,Marek Chadalski,Agnieszka Toma-Jonik,Natalia Vydra,Piotr Widłak,Bartosz Wojtaś,Bartłomiej Gielniewski,Wieslawa Widlak

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SPEN antibody

Immunoprecipitation - Anti-SPEN antibody (AB72266)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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