Rabbit Polyclonal Spermidine antibody. Suitable for IHC-P and reacts with Chemical samples. Cited in 7 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Spermidine.
Images
Publications
- Communications biology 6:6762023Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeishi Aihara,Kumiko Torisu,Yushi Uchida,Noriyuki Imazu,Toshiaki Nakano,Takanari KitazonoPubMed 37380734
- Biomedicines 11:2023Changes in the Localization of Polyamine Spermidine in the Rat Retina with Age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid S Ríos,Christian J Malpica-Nieves,Amanda Díaz-García,Misty J Eaton,Serguei N SkatchkovPubMed 37189626
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to Spermidine.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Chemical | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Target data
Recommended products
Rabbit Polyclonal Spermidine antibody. Suitable for IHC-P and reacts with Chemical samples. Cited in 7 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Spermidine.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to Spermidine.
- Purity
Whole antiserum
- Specificity
Reacts with spermidine and cross reacts with spermine.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
Spermidine also known as SPD is a polyamine with a molecular mass of approximately 145.25 g/mol. It originates from the decarboxylation product of putrescine and is synthesized in the cytoplasm. Spermidine expresses ubiquitously in both prokaryotic and eukaryotic cells. Its mechanical role involves stabilizing the structure of the ribosome and enhancing nucleic acid functions. Through its interaction with RNA it influences cellular functions like growth and proliferation.
- Biological function summary
This polyamine plays an important role in cellular processes such as autophagy where it acts as an important regulator. Spermidine is not part of a larger protein complex but it interacts directly with other cellular components to modulate cellular homeostasis and stress resistance. Additionally spermidine is involved in processes like telomerase activity and apoptosis proving its significance in cell maintenance and longevity.
- Pathways
Spermidine significantly contributes to the polyamine biosynthesis and catabolism pathways. It is involved in the regulation of the mechanistic target of rapamycin (mTOR) pathway which is important for controlling cellular growth and metabolism. Spermidine functions in connection with proteins like ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) which are pivotal for the polyamine synthesis pathway and interconnected processes.
- Associated diseases and disorders
Spermidine shows relevance to conditions such as neurodegenerative disorders and certain cancers. Its ability to modulate autophagy suggests its importance in mitigating the progression of neurodegenerative diseases like Alzheimer's. Additionally spermidine's role in cell growth regulation links it to cancer as dysregulated polyamine metabolism often correlates with tumorigenesis. In these conditions spermidine interacts with proteins such as Beclin 1 in autophagy and MYC in cell growth regulation highlighting its therapeutic potential in managing these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Image from Chung-Ping liao et. al. J. Biol. Chem., Mar 2009; 284: 8174 - 8184. (Fig 3B).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spermidine antibody (ab7318)
ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.
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