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AB111884

Anti-Spermidine synthase antibody

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(3 Publications)

Rabbit Polyclonal Spermidine synthase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRM aa 1-250.

View Alternative Names

SPS1, SRML1, SRM, Spermidine synthase, SPDSY, Putrescine aminopropyltransferase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spermidine synthase antibody (AB111884)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spermidine synthase antibody (AB111884)

ab111884 at 1/500 dilution staining Spermidine synthase in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

Western blot - Anti-Spermidine synthase antibody (AB111884)
  • WB

Unknown

Western blot - Anti-Spermidine synthase antibody (AB111884)

12% SDS PAGE

All lanes:

Western blot - Anti-Spermidine synthase antibody (ab111884) at 1/5000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SRM aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P19623

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>or Tris-EDTA buffer ( PH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111884 is purified by antigen-affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermidine synthase also known as SPDSY or SRM catalyzes the production of spermidine from putrescine. This enzymatic reaction involves decarboxylated S-adenosylmethionine and is an essential step in polyamine synthesis. Spermidine synthase is a well-characterized enzyme with a molecular mass of approximately 35 kDa. The enzyme is predominantly expressed in tissues with high cellular turnover and proliferation such as the liver kidney and testis.
Biological function summary

Spermidine synthase plays a critical role in the synthesis of polyamines such as spermine which are vital for cell growth and differentiation. Polyamines stabilize DNA structure modulate ion channels and aid in gene expression. Spermidine synthase operates as a standalone enzyme and is not part of a multi-protein complex. Its expression levels and activity are tightly regulated reflecting its importance in cellular homeostasis and response to stress.

Pathways

Spermidine synthase is central to the polyamine biosynthesis pathway which is important for cellular proliferation. In this pathway it acts after ornithine decarboxylase converting putrescine to spermidine and before spermine synthase which produces spermine from spermidine. Spermidine synthase is also involved in the regulation of eukaryotic translation initiation highlighting its influence on protein biosynthesis and cell growth.

Aberrant expression or activity of spermidine synthase has been linked to cancer and neurodegenerative diseases. In cancer elevated levels of polyamines can promote tumor growth and survival. The enzyme's activity can be influenced by proteins such as antizyme which regulates polyamine levels. In neurodegenerative disorders like Alzheimer's disease disrupted polyamine metabolism may contribute to neuronal dysfunction. Understanding spermidine synthase's role in these conditions helps elucidate the molecular mechanisms underlying disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.
See full target information SRM
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928162

2024

Spermidine Synthase Localization in Retinal Layers: Early Age Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid Zayas-Santiago,Christian J Malpica-Nieves,David S Ríos,Amanda Díaz-García,Paola N Vázquez,José M Santiago,David E Rivera-Aponte,Rüdiger W Veh,Miguel Méndez-González,Misty Eaton,Serguei N Skatchkov

Nature communications 10:3546 PubMed31391464

2019

Supramolecular trap for catching polyamines in cells as an anti-tumor strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Chen,Hanzhi Ni,Zhao Meng,Jing Wang,Xiayang Huang,Yansheng Dong,Chao Sun,Yadan Zhang,Lei Cui,Jian Li,Xueshun Jia,Qingbin Meng,Chunju Li

Oncotarget 7:14380-93 PubMed26910893

2016

The essential role of methylthioadenosine phosphorylase in prostate cancer.

Applications

WB

Species

Unspecified reactive species

Gaia Bistulfi,Hayley C Affronti,Barbara A Foster,Ellen Karasik,Bryan Gillard,Carl Morrison,James Mohler,James G Phillips,Dominic J Smiraglia
View all publications
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