Anti-Spermidine synthase antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Spermidine synthase antibody (AB241496)

Spermidine synthase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241496 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241496 at 0.4 μg/ml.

Lane 1 : ab241496 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-Spermidine synthase antibody (ab241496)

Predicted band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-Spermidine synthase antibody (AB241496)

All lanes:

Western blot - Anti-Spermidine synthase antibody (ab241496) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 30s