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AB26975

Anti-Spermine antibody

4

(3 Reviews)

|

(7 Publications)

Rabbit Polyclonal Spermine antibody. Suitable for ICC/IF, WB and reacts with Chemical samples. Cited in 7 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Spermine.
1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Spermine antibody (AB26975)
  • ICC/IF

AbReview20482****

Immunocytochemistry/ Immunofluorescence - Anti-Spermine antibody (AB26975)

ab26975 staining spermine in the Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde for 15 minuts at room temperature (Upper images) or iced methanol of 3 minutes (Lower images) and blocked with 2% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/100) for 1 hour at 27°C. An Alexa Fluor®568-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. Samples handled without ab26975 were used as negative controls (Left-hand panels).

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB, ICC/IF

applications

Specificity

<p>This antibody reacts with spermine. Cross-reacts with spermidine 100%.</p>

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Complementary Products

Primary Antibodies

AB7318

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Primary Antibodies

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  • 100
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB223287

Anti-FSTL1/FRP antibody [EPR21933-155]

RabMAb|Recombinant|KO Validated

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1200 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins.
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine is an organic polyamine compound with a molecular mass of 202.34 g/mol. It plays an essential role in cellular metabolism. Often found in eukaryotic cells spermine is also known as sym-norspermidine. The compound spreads across tissues with high concentrations in tissues that have rapid growth such as the thymus and testes. It exists intracellularly and consists of several amine groups which enable it to interact with negatively charged molecules like DNA and RNA. These interactions increase the stability and control of nucleic acids within the cellular environment.
Biological function summary

Spermine modulates various cellular processes. It is involved in cell growth and differentiation and it stabilizes cell membranes. By binding to DNA spermine helps preserve its structure during stress. Additionally spermine acts as an antioxidant reducing oxidative damage in the cells. Polyamines such as spermine often function independently rather than as part of a complex. The regulation of their synthesis and uptake is controlled by enzymes including ornithine decarboxylase which is an important player in polyamine biosynthesis.

Pathways

Spermine is central in the polyamine biosynthesis pathway which is vital for cellular proliferation. It regulates pathways involved in the synthesis and breakdown of proteins. The action of spermine interlinks with the metabolism of amino acids like ornithine and methionine influencing the biogenic amines pathway. Spermine's metabolic pathway intersects with proteins such as S-adenosylmethionine decarboxylase and spermidine synthase which closely control its levels in the cell.

Spermine has connections to several pathological conditions including cancer and neurodegenerative diseases. Its role in promoting cell growth links it to oncogenesis where abnormal regulation can lead to cancerous growths. Increased spermine levels associate with high-grade tumors. Likewise its oxidative stress modulation impacts neurodegenerative disorders like Parkinson's disease. The disruption in the polyamine pathway influencing spermine levels can exacerbate neuronal injury. Interactions with proteins such as N-methyl-D-aspartate (NMDA) receptors also highlight spermine's involvement in neurotoxicity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Spermine
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Radiation oncology (London, England) 20:138 PubMed40898344

2025

Comparative metabolomic analysis of human lung slices (hu-PCLS) exposed to either standard or FLASH protons: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia Velalopoulou,Tytus D Mak,Annabella Deziel,Michele M Kim,Constantinos Koumenis,Melpo Christofidou-Solomidou,Evagelia C Laiakis

PLoS pathogens 21:e1012501 PubMed40834051

2025

Daxx mediated histone H3.3 deposition on HSV-1 DNA restricts genome decompaction and the progression of immediate-early transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley P E Roberts,Anne Orr,Victor Iliev,Lauren Orr,Steven McFarlane,Zhousiyu Yang,Ilaria Epifano,Colin Loney,Mila Collados Rodriguez,Anna R Cliffe,Kristen L Conn,Chris Boutell

Frontiers in cellular neuroscience 15:667046 PubMed34393723

2021

Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Vila,Eyad Shihabeddin,Zhijing Zhang,Abirami Santhanam,Christophe P Ribelayga,John O'Brien

Proceedings of the National Academy of Sciences of 116:12540-12549 PubMed31152136

2019

ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes.

Applications

Unspecified application

Species

Unspecified reactive species

Thanh Ha Thi Do,Hyunju Choi,Michael Palmgren,Enrico Martinoia,Jae-Ung Hwang,Youngsook Lee

The Journal of biological chemistry 292:6369-6380 PubMed28258224

2017

Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish.

Applications

WB

Species

Unspecified reactive species

Kouki Maki,Toshio Shibata,Shun-Ichiro Kawabata

Nature communications 6:8532 PubMed26449415

2015

KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis.

Applications

Unspecified application

Species

Unspecified reactive species

Ken-Ichi Nakajima,Kan Zhu,Yao-Hui Sun,Bence Hegyi,Qunli Zeng,Christopher J Murphy,J Victor Small,Ye Chen-Izu,Yoshihiro Izumiya,Josef M Penninger,Min Zhao

Plant physiology 152:1842-50 PubMed20147493

2010

Crystal structure and functional insights of hemopexin fold protein from grass pea.

Applications

Unspecified application

Species

Unspecified reactive species

Vineet Gaur,Insaf A Qureshi,Apekshita Singh,Veenu Chanana,Dinakar M Salunke
View all publications
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Product promise

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