AB101458

Anti-Spermine synthase antibody

Name: Anti-Spermine synthase antibody (ab101458) | Abcam
Brand: Abcam Limited
SKU: AB101458
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal Spermine synthase antibody. Suitable for WB and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Spermine synthase aa 100-350.

View Alternative Names

Spermine synthase, SPMSY, Spermidine aminopropyltransferase, SMS

2 Images

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Western blot - Anti-Spermine synthase antibody (AB101458)

10% SDS-PAGE

All lanes:

Western blot - Anti-Spermine synthase antibody (ab101458) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

Jurkat whole cell lysate at 30 µg

Predicted band size: 104 kDa,116 kDa,127 kDa,128 kDa,139 kDa,141 kDa,143 kDa,149 kDa,158 kDa,175 kDa,18 kDa,20 kDa,21 kDa,23 kDa,24 kDa,25 kDa,31 kDa,32 kDa,33 kDa,35 kDa,36 kDa,37 kDa,38 kDa,39 kDa,41 kDa,42 kDa,43 kDa,47 kDa,49 kDa,52 kDa,53 kDa,55 kDa,58 kDa,59 kDa,60 kDa,61 kDa,63 kDa,64 kDa,66 kDa,7 kDa,72 kDa,73 kDa,74 kDa,76 kDa,82 kDa,83 kDa,84 kDa,96 kDa,97 kDa,98 kDa

Observed band size: 100 kDa,110 kDa,120 kDa,120-140 kDa,128 kDa,130 kDa,130-140 kDa,140 kDa,15 kDa,150 kDa,155 kDa,175 kDa,21 kDa,212 kDa,23 kDa,25 kDa,32 kDa,35 kDa,36 kDa,39 kDa,42 kDa,42-43 kDa,46 kDa,55 kDa,59 kDa,60 kDa,61 kDa,62 kDa,64 kDa,66 kDa,66-70 kDa,7 kDa,70-75 kDa,72 kDa,73 kDa,74 kDa,75 kDa,84 kDa,85 kDa,97 kDa

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Supplier Data

Western blot - Anti-Spermine synthase antibody (AB101458)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Spermine synthase antibody (ab101458) at 1/500 dilution

All lanes:

Rat kidney tissue extract at 50 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human Spermine synthase aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

P52788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine synthase also recognized as SMS is an enzyme that catalyzes the conversion of spermidine to spermine. Mechanically it transfers an aminopropyl group from decarboxylated S-adenosylmethionine to spermidine resulting in spermine. This enzyme is around 43 kDa in size and is ubiquitously expressed in various tissues with notable expression in the liver and kidney. It plays an essential role in the polyamine biosynthetic pathway contributing to cellular growth and differentiation.

Biological function summary

The enzyme fosters cellular functions by modulating spermine levels which are necessary for stabilizing DNA RNA and ribosome structures. It does not exist as part of a larger complex but works closely with other enzymes in the polyamine synthesis process. Spermine contributes to cellular homeostasis influencing ion channel activity and defending against oxidative stress. The balanced production of spermine by spermine synthase represents its importance in maintaining normal cellular physiology.

Pathways

Spermine synthase plays an important role in the polyamine metabolic pathway converting spermidine to spermine finalizing the chain of polyamine biosynthesis. It interacts closely with enzymes like ornithine decarboxylase which facilitates the initial steps of polyamine synthesis and spermidine synthase which precedes spermine formation. The pathway is fundamental to cell proliferation apoptosis and differentiation processes emphasizing the enzyme's pivotal part in cell biology.

Alterations in spermine synthase activity associate with Snyder-Robinson syndrome and cancer. Snyder-Robinson syndrome connected to mutations in the SMS gene results in neurodevelopmental difficulties and skeletal abnormalities. In cancer dysregulation of polyamine synthesis where spermine synthase is implicated contributes to uncontrolled cellular proliferation. Changes in spermine levels impact other proteins including ornithine decarboxylase and other polyamine pathway enzymes which participate in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).

See full target information Spermine synthase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 61:9 PubMed32049341

2020

Increased Rate of Retinal Pigment Epithelial Cell Migration and Pro-Angiogenic Potential Ensuing From Reduced Cystatin C Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Emil Carlsson,Wasu Supharattanasitthi,Malcolm Jackson,Luminita Paraoan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spermine synthase antibody

Western blot - Anti-Spermine synthase antibody (AB101458)

Western blot - Anti-Spermine synthase antibody (AB101458)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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