AB156879

Anti-Spermine synthase antibody [EPR9252(B)]

RabMAb
Recombinant
KO Validated
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(10 Publications)

Rabbit Recombinant Monoclonal Spermine synthase antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

Spermine synthase, SPMSY, Spermidine aminopropyltransferase, SMS

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.

Lab

Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Spermine synthase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

Predicted band size: 41 kDa

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Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution

Lane 1:

Human fetal heart extract at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 41 kDa

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Carrier free

Anti-Spermine synthase antibody [EPR9252(B)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Spermine synthase antibody [EPR9252(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Spermine synthase antibody [EPR9252(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Spermine synthase antibody [EPR9252(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Spermine synthase antibody [EPR9252(B)]
660 APC

APC Anti-Spermine synthase antibody [EPR9252(B)]
578 PE

PE Anti-Spermine synthase antibody [EPR9252(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9252(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine synthase also recognized as SMS is an enzyme that catalyzes the conversion of spermidine to spermine. Mechanically it transfers an aminopropyl group from decarboxylated S-adenosylmethionine to spermidine resulting in spermine. This enzyme is around 43 kDa in size and is ubiquitously expressed in various tissues with notable expression in the liver and kidney. It plays an essential role in the polyamine biosynthetic pathway contributing to cellular growth and differentiation.

Biological function summary

The enzyme fosters cellular functions by modulating spermine levels which are necessary for stabilizing DNA RNA and ribosome structures. It does not exist as part of a larger complex but works closely with other enzymes in the polyamine synthesis process. Spermine contributes to cellular homeostasis influencing ion channel activity and defending against oxidative stress. The balanced production of spermine by spermine synthase represents its importance in maintaining normal cellular physiology.

Pathways

Spermine synthase plays an important role in the polyamine metabolic pathway converting spermidine to spermine finalizing the chain of polyamine biosynthesis. It interacts closely with enzymes like ornithine decarboxylase which facilitates the initial steps of polyamine synthesis and spermidine synthase which precedes spermine formation. The pathway is fundamental to cell proliferation apoptosis and differentiation processes emphasizing the enzyme's pivotal part in cell biology.

Alterations in spermine synthase activity associate with Snyder-Robinson syndrome and cancer. Snyder-Robinson syndrome connected to mutations in the SMS gene results in neurodevelopmental difficulties and skeletal abnormalities. In cancer dysregulation of polyamine synthesis where spermine synthase is implicated contributes to uncontrolled cellular proliferation. Changes in spermine levels impact other proteins including ornithine decarboxylase and other polyamine pathway enzymes which participate in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).

See full target information Spermine synthase

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7827 PubMed40846845

2025

Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hanshen Yang,Xiaozhen Zhang,Sirui Zhang,Yanqing Yang,Yan Chen,Yangwei Jiang,Qingsong Lu,Lingyue Liu,Mengyi Lao,Weiran Du,Kang Sun,Lihong He,Jiatao Shi,Xinyuan Liu,Jinyuan Song,Na Lu,Junming Huang,Jinyan Huang,Ruhong Zhou,Xiongbin Lu,Tingbo Liang,Xueli Bai

Frontiers in oncology 15:1613458 PubMed40823069

2025

Polyamine metabolism related gene index prediction of prognosis and immunotherapy response in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoya Wang,Shouliang Cai,Qing Gao,Yidong Chen,Xue Han,Fangjian Shang,Chunyan Liang,Guolian Zhu,Bo Chen

Journal of Korean Neurosurgical Society 68:75-96 PubMed39492653

2024

Spermine Synthase : A Potential Prognostic Marker for Lower-Grade Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Liu,Hongqi Li,Xiaolong Hu,Maohui Yan,Zhiguang Fu,Hengheng Zhang,Yingjie Wang,Nan Du

Cell death & disease 15:573 PubMed39117605

2024

Elucidating the role of S100A10 in CD8 T cell exhaustion and HCC immune escape via the cPLA2 and 5-LOX axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ganggang Wang,Xiaowei Shen,Wenzhi Jin,Chao Song,Meiyuan Dong,Zhijie Zhou,Xiaoliang Wang

Disease models & mechanisms 17: PubMed38463005

2024

Impaired polyamine metabolism causes behavioral and neuroanatomical defects in a mouse model of Snyder-Robinson syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Oluwaseun Akinyele,Anushe Munir,Marie A Johnson,Megan S Perez,Yuan Gao,Jackson R Foley,Ashley Nwafor,Yijen Wu,Tracy Murray-Stewart,Robert A Casero,Hülya Bayir,Dwi U Kemaladewi

Translational andrology and urology 12:1497-1510 PubMed37969768

2023

Proteomic approach towards identification of seminal fluid biomarkers from individuals with severe oligozoospermia, cryptozoospermia and non-obstructive azoospermia: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Nowicka-Bauer,Marzena Kamieniczna,Marta Olszewska,Maciej Krzysztof Kurpisz

The Journal of clinical investigation 131: PubMed33586680

2021

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie A Sandusky-Beltran,Andrii Kovalenko,Devon S Placides,Kevin Ratnasamy,Chao Ma,Jerry B Hunt,Huimin Liang,John Ivan T Calahatian,Camilla Michalski,Margaret Fahnestock,Laura J Blair,April L Darling,Jeremy D Baker,Sarah N Fontaine,Chad A Dickey,Joshua J Gamsby,Kevin R Nash,Erin Abner,Maj-Linda B Selenica,Daniel C Lee

Nature communications 10:3546 PubMed31391464

2019

Supramolecular trap for catching polyamines in cells as an anti-tumor strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Chen,Hanzhi Ni,Zhao Meng,Jing Wang,Xiayang Huang,Yansheng Dong,Chao Sun,Yadan Zhang,Lei Cui,Jian Li,Xueshun Jia,Qingbin Meng,Chunju Li

JCI insight 3: PubMed29997303

2018

Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Sungsam Gong,Ulla Sovio,Irving Lmh Aye,Francesca Gaccioli,Justyna Dopierala,Michelle D Johnson,Angela M Wood,Emma Cook,Benjamin J Jenkins,Albert Koulman,Robert A Casero,Miguel Constância,D Stephen Charnock-Jones,Gordon Cs Smith

Oncotarget 7:14380-93 PubMed26910893

2016

The essential role of methylthioadenosine phosphorylase in prostate cancer.

Applications

Species

Unspecified reactive species

Gaia Bistulfi,Hayley C Affronti,Barbara A Foster,Ellen Karasik,Bryan Gillard,Carl Morrison,James Mohler,James G Phillips,Dominic J Smiraglia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spermine synthase antibody [EPR9252(B)]

Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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