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AB240139

Anti-Spermine synthase antibody [EPR9252(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Spermine synthase antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Spermine synthase, SPMSY, Spermidine aminopropyltransferase, SMS

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] - BSA and Azide free (AB240139)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] - BSA and Azide free (AB240139)

Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156879).

  • Unconjugated

    Anti-Spermine synthase antibody [EPR9252(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Spermine synthase antibody [EPR9252(B)]

  • 660 APC

    APC Anti-Spermine synthase antibody [EPR9252(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Spermine synthase antibody [EPR9252(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Spermine synthase antibody [EPR9252(B)]

  • 578 PE

    PE Anti-Spermine synthase antibody [EPR9252(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Spermine synthase antibody [EPR9252(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9252(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab240139 is the carrier-free version of ab156879.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine synthase also recognized as SMS is an enzyme that catalyzes the conversion of spermidine to spermine. Mechanically it transfers an aminopropyl group from decarboxylated S-adenosylmethionine to spermidine resulting in spermine. This enzyme is around 43 kDa in size and is ubiquitously expressed in various tissues with notable expression in the liver and kidney. It plays an essential role in the polyamine biosynthetic pathway contributing to cellular growth and differentiation.
Biological function summary

The enzyme fosters cellular functions by modulating spermine levels which are necessary for stabilizing DNA RNA and ribosome structures. It does not exist as part of a larger complex but works closely with other enzymes in the polyamine synthesis process. Spermine contributes to cellular homeostasis influencing ion channel activity and defending against oxidative stress. The balanced production of spermine by spermine synthase represents its importance in maintaining normal cellular physiology.

Pathways

Spermine synthase plays an important role in the polyamine metabolic pathway converting spermidine to spermine finalizing the chain of polyamine biosynthesis. It interacts closely with enzymes like ornithine decarboxylase which facilitates the initial steps of polyamine synthesis and spermidine synthase which precedes spermine formation. The pathway is fundamental to cell proliferation apoptosis and differentiation processes emphasizing the enzyme's pivotal part in cell biology.

Alterations in spermine synthase activity associate with Snyder-Robinson syndrome and cancer. Snyder-Robinson syndrome connected to mutations in the SMS gene results in neurodevelopmental difficulties and skeletal abnormalities. In cancer dysregulation of polyamine synthesis where spermine synthase is implicated contributes to uncontrolled cellular proliferation. Changes in spermine levels impact other proteins including ornithine decarboxylase and other polyamine pathway enzymes which participate in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).
See full target information Spermine synthase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2712:157-163 PubMed37578704

2023

In-Cell Western Assay in Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Wang,Jingbo Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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