AB151547

Anti-Spermine synthase antibody [EPR9253]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Spermine synthase antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Spermine synthase, SPMSY, Spermidine aminopropyltransferase, SMS

3 Images

Flow Cytometry (Intracellular) - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Spermine synthase (red) with ab151547 at 1/10 dilution, compared to a nonspecific control antibody (green).

Lab

Western blot - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Spermine synthase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab151547 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab151547 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab151547 and ab18058 (loading control to vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547)

Predicted band size: 41 kDa

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Western blot - Anti-Spermine synthase antibody [EPR9253] (AB151547)

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa

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Carrier free

Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9253

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine synthase also recognized as SMS is an enzyme that catalyzes the conversion of spermidine to spermine. Mechanically it transfers an aminopropyl group from decarboxylated S-adenosylmethionine to spermidine resulting in spermine. This enzyme is around 43 kDa in size and is ubiquitously expressed in various tissues with notable expression in the liver and kidney. It plays an essential role in the polyamine biosynthetic pathway contributing to cellular growth and differentiation.

Biological function summary

The enzyme fosters cellular functions by modulating spermine levels which are necessary for stabilizing DNA RNA and ribosome structures. It does not exist as part of a larger complex but works closely with other enzymes in the polyamine synthesis process. Spermine contributes to cellular homeostasis influencing ion channel activity and defending against oxidative stress. The balanced production of spermine by spermine synthase represents its importance in maintaining normal cellular physiology.

Pathways

Spermine synthase plays an important role in the polyamine metabolic pathway converting spermidine to spermine finalizing the chain of polyamine biosynthesis. It interacts closely with enzymes like ornithine decarboxylase which facilitates the initial steps of polyamine synthesis and spermidine synthase which precedes spermine formation. The pathway is fundamental to cell proliferation apoptosis and differentiation processes emphasizing the enzyme's pivotal part in cell biology.

Alterations in spermine synthase activity associate with Snyder-Robinson syndrome and cancer. Snyder-Robinson syndrome connected to mutations in the SMS gene results in neurodevelopmental difficulties and skeletal abnormalities. In cancer dysregulation of polyamine synthesis where spermine synthase is implicated contributes to uncontrolled cellular proliferation. Changes in spermine levels impact other proteins including ornithine decarboxylase and other polyamine pathway enzymes which participate in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).

See full target information Spermine synthase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 20:100115 PubMed34129943

2021

Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqiu Gong,Yu Liu,Tian Wang,Zhigui Li,Li Gao,Haining Chen,Yang Shu,Yuan Li,Heng Xu,Zongguang Zhou,Lunzhi Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spermine synthase antibody [EPR9253]

Flow Cytometry (Intracellular) - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Western blot - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Western blot - Anti-Spermine synthase antibody [EPR9253] (AB151547)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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