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AB248996

Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Spermine synthase antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Spermine synthase, SPMSY, Spermidine aminopropyltransferase, SMS

3 Images
Flow Cytometry (Intracellular) - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)

This data was developed using ab151547, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Spermine synthase (red) with ab151547 at 1/10 dilution, compared to a nonspecific control antibody (green).

Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)
  • WB

Unknown

Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)

This data was developed using ab151547, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9253] (<a href='/en-us/products/primary-antibodies/spermine-synthase-antibody-epr9253-ab151547'>ab151547</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa

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Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)
  • WB

Lab

Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (AB248996)

This data was developed using ab151547, the same antibody clone in a different buffer formulation.

Lane 1 Wild-type HAP1 cell lysate (20 μg)

Lane 2 Spermine synthase knockout HAP1 cell lysate (20 μg)

Lane 3 HeLa cell lysate (20 μg)

Lanes 1 - 3 Merged signal (red and green). Green - ab151547 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab151547 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab151547 and ab18058 (loading control to vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Spermine synthase antibody [EPR9253] (<a href='/en-us/products/primary-antibodies/spermine-synthase-antibody-epr9253-ab151547'>ab151547</a>)

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9253

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB97395

Anti-Ornithine Decarboxylase/ODC antibody

Western blot - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB241496

Anti-Spermidine synthase antibody

Immunoprecipitation - Anti-Spermidine synthase antibody (AB241496)

  • 0
  • 0
Primary Antibodies

AB32443

Anti-eIF5A antibody [EP526Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-eIF5A antibody [EP526Y] (AB32443)

  • 4
  • 1
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Reactivity data

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Product details

ab248996 is the carrier-free version of ab151547.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spermine synthase also recognized as SMS is an enzyme that catalyzes the conversion of spermidine to spermine. Mechanically it transfers an aminopropyl group from decarboxylated S-adenosylmethionine to spermidine resulting in spermine. This enzyme is around 43 kDa in size and is ubiquitously expressed in various tissues with notable expression in the liver and kidney. It plays an essential role in the polyamine biosynthetic pathway contributing to cellular growth and differentiation.
Biological function summary

The enzyme fosters cellular functions by modulating spermine levels which are necessary for stabilizing DNA RNA and ribosome structures. It does not exist as part of a larger complex but works closely with other enzymes in the polyamine synthesis process. Spermine contributes to cellular homeostasis influencing ion channel activity and defending against oxidative stress. The balanced production of spermine by spermine synthase represents its importance in maintaining normal cellular physiology.

Pathways

Spermine synthase plays an important role in the polyamine metabolic pathway converting spermidine to spermine finalizing the chain of polyamine biosynthesis. It interacts closely with enzymes like ornithine decarboxylase which facilitates the initial steps of polyamine synthesis and spermidine synthase which precedes spermine formation. The pathway is fundamental to cell proliferation apoptosis and differentiation processes emphasizing the enzyme's pivotal part in cell biology.

Alterations in spermine synthase activity associate with Snyder-Robinson syndrome and cancer. Snyder-Robinson syndrome connected to mutations in the SMS gene results in neurodevelopmental difficulties and skeletal abnormalities. In cancer dysregulation of polyamine synthesis where spermine synthase is implicated contributes to uncontrolled cellular proliferation. Changes in spermine levels impact other proteins including ornithine decarboxylase and other polyamine pathway enzymes which participate in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).
See full target information Spermine synthase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 184:4186-4202.e20 PubMed34216540

2021

Polyamine metabolism is a central determinant of helper T cell lineage fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Puleston,Francesc Baixauli,David E Sanin,Joy Edwards-Hicks,Matteo Villa,Agnieszka M Kabat,Marcin M Kamiński,Michal Stanckzak,Hauke J Weiss,Katarzyna M Grzes,Klara Piletic,Cameron S Field,Mauro Corrado,Fabian Haessler,Chao Wang,Yaarub Musa,Lena Schimmelpfennig,Lea Flachsmann,Gerhard Mittler,Nir Yosef,Vijay K Kuchroo,Joerg M Buescher,Stefan Balabanov,Edward J Pearce,Douglas R Green,Erika L Pearce
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com