AB243570

Anti-SPERT antibody

Name: Anti-SPERT antibody (ab243570) | Abcam
Brand: Abcam Limited
SKU: AB243570
Price: 630 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal SPERT antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBY2 aa 50-200.

View Alternative Names

SPERT, CBY2, Protein chibby homolog 2, Spermatid-associated protein

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Formalin-fixed, paraffin-embedded human testis tissue stained for SPERT with ab243570 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SPERT with ab243570 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Formalin-fixed, paraffin-embedded human colon tissue stained for SPERT with ab243570 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for SPERT with ab243570 at 1/2500 dilution in immunohistochemical analysis. Low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-SPERT antibody (AB243570)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPERT antibody (AB243570)

PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling SPERT using ab243570 at 4 μg/ml (green) in ICC/IF. Microtubules in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Formalin-fixed, paraffin-embedded human liver tissue stained for SPERT with ab243570 at 1/2500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CBY2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q8NA61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPERT also known as sperm-associated antigen 9 (SPAG9) weighs approximately 40 kDa. This protein mainly finds expression in testis tissue but also appears in other tissues like breast and prostate albeit at lower levels. SPERT localizes in the cytoplasm and nucleus of cells suggesting its role in various cellular processes.

Biological function summary

SPERT interacts with components critical for spermatogenesis and fertilization processes. It forms part of a protein complex that regulates specific functions during sperm development. Through its associations SPERT influences microtubule binding and possibly affects the integrity and motility of sperm cells. These functions highlight its important role in male fertility.

Pathways

Researchers connect SPERT to the JNK signaling pathway which regulates stress responses and apoptosis. In this context SPERT modulates interactions with proteins like c-Jun N-terminal kinases (JNKs) playing a role in cell survival and apoptosis. SPERT’s involvement in regulating kinase activity links it to MAPK pathways which further emphasize its influence on cellular growth and differentiation.

SPERT has been implicated in certain reproductive disorders such as male infertility. Abnormal expression or mutations in SPERT may impact sperm function linked to disrupted spermatogenesis. Additionally irregular SPERT expression correlates with breast cancer possibly through its interactions affecting cellular pathways driven by JNK and MAPK proteins. Understanding these links helps recognize SPERT's role in pathological conditions and may guide future therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CBY2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 13:1581019 PubMed40453182

2025

Development of a procedure for isolation, identification and quality assessment of bovine spermatids and evaluation of their fertilizing ability .

Applications

Unspecified application

Species

Unspecified reactive species

Rolando Pasquariello,Francesca Di Filippo,Sai Kamal Nag Bonumallu,Fernanda Fagali Franchi,Ramona Pistucci,Federica Franciosi,Valentina Lodde,Alessandra Iannuzzi,Alberto Maria Luciano,Tiziana A L Brevini,Fulvio Gandolfi

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPERT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Immunocytochemistry/ Immunofluorescence - Anti-SPERT antibody (AB243570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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