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AB313498

Anti-SPG11 antibody [RP23040242]

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Rabbit Recombinant Monoclonal SPG11 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPG11.

View Alternative Names

KIAA1840, SPG11, Spatacsin, Colorectal carcinoma-associated protein, Spastic paraplegia 11 protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPG11 Antibody [RP23040242] (AB313498)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPG11 Antibody [RP23040242] (AB313498)

Immunofluorescent analysis of SH-SY5Y cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous Spatacsin with ab313498 at 5 ?g/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Spatacsin (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Spatacsin. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Western blot - Anti-SPG11 Antibody [RP23040242] (AB313498)
  • WB

Supplier Data

Western blot - Anti-SPG11 Antibody [RP23040242] (AB313498)

All lanes:

Western blot - Anti-SPG11 Antibody [RP23040242] (ab313498) at 1 µg/mL

Lane 1:

Untransfected HeLa cell lysate

Lane 2:

non-specific scrambled siRNA transfected HeLa cell lysate

Lane 3:

Spatacsin specific siRNA transfected HeLa cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 278 kDa

false

Western blot - Anti-SPG11 Antibody [RP23040242] (AB313498)
  • WB

Supplier Data

Western blot - Anti-SPG11 Antibody [RP23040242] (AB313498)

All lanes:

Western blot - Anti-SPG11 Antibody [RP23040242] (ab313498) at 2.5 µg/mL

Lane 1:

SH-SY5Y cell lysate at 30 µg

Lane 2:

U87MG cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 278 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SPG11.

Q96JI7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPG11 also known as spatacsin is a protein with a mass of approximately 249 kDa. This protein is expressed mainly in neural tissues including the brain and spinal cord. Mechanically SPG11 interacts with various other proteins contributing to the maintenance and function of the lysosomal compartment within cells. It plays an important role in membrane trafficking processes particularly in the vesicles involved in endocytosis and autophagy.
Biological function summary

In the context of neuronal health SPG11 is significant for the function of neurons. It forms part of a larger protein complex known as the AP-5 adaptin complex which assists in lysosomal positioning and function. By ensuring proper lysosomal function SPG11 supports neuronal maintenance and health. Issues with its function disrupt lysosomal degradation pathways and can have broad implications for neuron viability.

Pathways

SPG11 is essential in endolysosomal and autophagic pathways serving as a connection point for effective lysosomal transport. These pathways include interactions with proteins such as AP5Z1 and ZFYVE26 which collaborate to modulate lysosomal positioning and maintenance. SPG11's proper operation within these pathways allows for efficient cellular digestion of macromolecules and removal of damaged organelles critical for cellular health.

Mutations in SPG11 lead to rare neurological conditions like spastic paraplegia type 11 and some forms of juvenile amyotrophic lateral sclerosis (ALS). SPG11's mutation or dysfunction can result in improper lysosomal degradation contributing to the nervous system's degeneration. Related proteins like spastizin (ZFYVE26) and AP5Z1 when disrupted also exhibit similar symptoms of neural decay highlighting SPG11's integral role in neuronal disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in neurite plasticity by maintaining cytoskeleton stability and regulating synaptic vesicle transport.
See full target information SPG11
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Product promise

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