AB220229

Anti-SPG21/Maspardin antibody

Name: Anti-SPG21/Maspardin antibody (ab220229) | Abcam
Brand: Abcam Limited
SKU: AB220229
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SPG21/Maspardin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPG21 aa 200-350.

View Alternative Names

ACP33, BM-019, GL010, SPG21, Maspardin, Acid cluster protein 33, Spastic paraplegia 21 autosomal recessive Mast syndrome protein, Spastic paraplegia 21 protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SPG21/Maspardin antibody (AB220229)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPG21/Maspardin antibody (AB220229)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A549 cells labeling SPG21/Maspardin with ab220229 at 4 μg/ml (green).

Supplier Data

Western blot - Anti-SPG21/Maspardin antibody (AB220229)

All lanes:

Western blot - Anti-SPG21/Maspardin antibody (ab220229) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPG21 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q9NZD8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPG21 also known as Maspardin is a protein involved in cellular functions with a molecular mass of approximately 48 kDa. It is widely expressed in human tissues notably in the brain and peripheral blood cells. The protein localizes mainly in the cytoplasm and connects with lipid membrane structures. Its mechanical role involves interactions with proteins in the trans-Golgi network and possibly modifies the transport functions within cells.

Biological function summary

SPG21 plays a role in cellular processes such as membrane trafficking and lysosomal function. It is a part of the protein complex involved in sorting and degrading membrane-bound proteins and lipids. Through its interactions with other proteins SPG21 helps maintain cellular homeostasis and protects cells from stress-related damage. It is sometimes associated with lysosome function regulation which is fundamental to cellular waste management.

Pathways

SPG21 interacts with molecules in the lysosomal and Golgi-related pathways. It fits into these pathways by assisting in the transport and breakdown of lysosomal enzymes. These processes are essential for cellular waste processing and lipid metabolism. SPG21 takes part in pathways with proteins like CLN3 and members of the Golgi complex sustaining cellular metabolic balance and facilitating protein recycling.

SPG21 connects closely with hereditary spastic paraplegia (HSP) and neurodegenerative disorders. Mutations in the SPG21 gene lead to a form of HSP known as Mast syndrome characterized by spasticity and neural degradation. SPG21 has potential links with proteins involved in neurodegeneration like SPG7 where dysfunctions can trigger similar symptomatic progressions. Understanding SPG21's role in these diseases aids in targeting therapeutic strategies to mitigate neurological impacts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role as a negative regulatory factor in CD4-dependent T-cell activation.

See full target information SPG21

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:105620 PubMed36465115

2022

TWIK-related acid-sensitive K channel 2 promotes renal fibrosis by inducing cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Jing Chen,Yufei Lu,Yan Yang,Weize Chen,Bo Shen,Jiachang Hu,Ping Jia,Sujuan Xu,Yiqin Shi,Yichun Ning,Jialin Wang,Yi Fang,Shuan Zhao,Yang Li,Yan Dai,Xiaoyan Zhang,Meng Xiang,Yang Tian,Zhichao Liu,Nana Song,Xiaoqiang Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPG21/Maspardin antibody

Immunocytochemistry/ Immunofluorescence - Anti-SPG21/Maspardin antibody (AB220229)

Western blot - Anti-SPG21/Maspardin antibody (AB220229)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise