AB233646

Anti-SPG3A/ATL1 antibody [1F6B12]

Name: Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) | Abcam
Brand: Abcam Limited
SKU: AB233646

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(2 Publications)

Mouse Monoclonal SPG3A/ATL1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATL1 aa 1-100.

View Alternative Names

GBP3, SPG3, SPG3A, ATL1, Atlastin-1, ATL-1, Brain-specific GTP-binding protein, GTP-binding protein 3, Guanine nucleotide-binding protein 3, Spastic paraplegia 3 protein A, GBP-3, hGBP3

2 Images

Supplier Data

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Expected MW is 41kDa.

All lanes:

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution

All lanes:

Recombinant human SPG3A/ATL1 protein.

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

All lanes:

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

SPG3A/ATL1 (aa 1-100)-hlgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 64 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F6B12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ATL1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8WXF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPG3A also known as Atlastin-1 or ATL1 is a GTPase enzyme with a molecular mass of approximately 64 kDa. This protein is highly important in the mechanical process of membrane trafficking and the maintenance of the endoplasmic reticulum (ER) network. Atlastin-1 is expressed predominantly in neurons especially in the cerebral cortex and the hippocampus where it plays a role in neural function.

Biological function summary

Atlastin-1 is involved in the fusion of ER membranes contributing to the network's tubular structure. The protein facilitates the dimerization and fusion of ER membranes a function that is fundamental for creating a continuous and smooth ER network. While it does not form part of a large protein complex its role in membrane dynamics involves interaction with other proteins that maintain ER structure and function.

Pathways

Atlastin-1 participates in the ER and Golgi-related pathways essential for protein trafficking and cellular homeostasis. It has significant interactions in these pathways with proteins like REEP1 and spastin both of which assist in maintaining the dynamic structure of the ER. These interactions underline Atlastin-1's contribution to vesicle transport and membrane fusion processes.

Mutations in the Atlastin-1 gene can lead to hereditary spastic paraplegia (HSP) a group of inherited disorders characterized by weakness and stiffness of the legs. This protein interacts in the context of HSP with proteins like spastin both of which are implicated in similar neuronal membrane dynamics disorders. Another condition linked to Atlastin-1 dysregulation is neuropathy where the protein's failure to maintain ER integrity can contribute to neuronal degradation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atlastin-1 (ATL1) is a membrane-anchored GTPase that mediates the GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network. It facilitates the formation of three-way junctions where ER tubules intersect (PubMed : 14506257, PubMed : 18270207, PubMed : 19665976, PubMed : 27619977, PubMed : 34817557, PubMed : 38509071). Two atlastin-1 on neighboring ER tubules bind GTP and form loose homodimers through the GB1/RHD3-type G domains and 3HB regions. Upon GTP hydrolysis, the 3HB regions tighten, pulling the membranes together to drive their fusion. After fusion, the homodimer disassembles upon release of inorganic phosphate (Pi). Subsequently, GDP dissociates, resetting the monomers to a conformation ready for a new fusion cycle (PubMed : 14506257, PubMed : 21220294, PubMed : 21368113, PubMed : 23334294, PubMed : 38509071). May also regulate more or less directly Golgi biogenesis (PubMed : 17321752). Indirectly regulates axonal development (By similarity).

See full target information ATL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23237 PubMed36193555

2022

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanli Zhang,Yan Feng,Min Wang,Xin Liu

Cancer immunology, immunotherapy : CII 71:1075-1091 PubMed34562123

2021

LncRNA MIR155HG induces M2 macrophage polarization and drug resistance of colorectal cancer cells by regulating ANXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhou,Jian Li,Mingmei Liao,Qi Zhang,Mei Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SPG3A/ATL1 antibody [1F6B12]

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise