AB305255

Anti-SPG7/PGN antibody [EPR26225-9]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal SPG7/PGN antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

CAR, CMAR, PGN, SPG7, Mitochondrial inner membrane m-AAA protease component paraplegin, Cell matrix adhesion regulator, Paraplegin, Spastic paraplegia 7 protein

7 Images

Supplier Data

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

SPG7/PGN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg with ab305255 at 1/30 dilution (2μg in 0.35mg lysates). VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg Lane 2 : ab305255 IP in HEK-293 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305255 in HEK-293 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (ab305255) at 1/30 dilution

All lanes:

HEK-293 (human embryonic kidney epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 88 kDa

false

Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

SPG7/PGN was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg with ab305255 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305255. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg

Lane 2 : ab305255 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305255 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (ab305255) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 88 kDa

false

Exposure time: 32s

Supplier Data

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 26 seconds

All lanes:

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (ab305255) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg

Lane 2:

HeLa mitochondria fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 88 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-SPG7 (ab305255) staining at 1/200000 dilution. Exposure time : 59 seconds.

All lanes:

Western blot - Anti-CHCHD10 antibody [EPR26966-33] (<a href='/en-us/products/primary-antibodies/chchd10-antibody-epr26966-33-ab308510'>ab308510</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg

Lane 2:

HeLa mitochondrial fraction at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 minutes

All lanes:

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (ab305255) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 3:

MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 6:

Mouse cerebellum tissue lysate at 20 µg

Lane 7:

Mouse heart tissue lysate at 20 µg

Lane 8:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 88 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 minutes

All lanes:

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (ab305255) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with siRNA specifically targeting SPG7/PGN, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 88 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. This blot was developed using a high sensitivity ECL substrate. In Western blot, anti-SPG7 antibody (ab305255) staining at 1/1000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds.

All lanes:

Western blot - Anti-CHCHD10 antibody [EPR26493-17] (<a href='/en-us/products/primary-antibodies/chchd10-antibody-epr26493-17-ab308508'>ab308508</a>) at 1/1000 dilution

Lane 1:

Mouse liver non-mitochondrial fraction at 20 µg

Lane 2:

Mouse liver mitochondria fraction at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

Exposure time: 92s

Carrier free

Anti-SPG7/PGN antibody [EPR26225-9] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26225-9

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPG7 also known as paraplegin is a mitochondrial metalloprotease. It has a molecular mass of approximately 88 kDa. SPG7 is encoded by the SPG7 gene located in humans on chromosome 16. It expresses widely in tissues with a strong presence in the nervous system and muscles. Alternate names for this protein are PGN and spastic paraplegia 7 due to its association with specific neurodegenerative conditions.

Biological function summary

SPG7 functions as a part of the mitochondrial inner membrane m-AAA protease complex. This complex performs essential roles in the maintenance of mitochondrial homeostasis by degrading damaged or misfolded proteins within the mitochondria. The presence of SPG7 is important for proper mitochondrial function. The protein has proteolytic activity which supports mitochondrial respiratory chain complexes' assembly and stability.

Pathways

SPG7 directly involves itself in mitochondrial protein quality control and the broader regulation of mitochondrial function. It interacts closely with ATP-dependent proteases like AFG3L2 playing a significant part in mitochondrial biogenesis pathways. These partnerships are key for maintaining cellular energy balance and ensuring efficient electron transport chain operation.

SPG7 mutations connect predominantly to hereditary spastic paraplegia (HSP) and have also been linked to ataxia. These mutations interrupt normal mitochondrial function leading to the degeneration of motor pathways. In HSP SPG7 interacts with proteins such as paraplegin-associated proteins SPG5 and SPG11 contributing to the disorder's pathogenesis. Understanding SPG7’s role in these diseases aids in exploring therapeutic strategies for mitochondrial-related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development (PubMed : 11549317, PubMed : 28396416, PubMed : 31097542, PubMed : 9635427). SPG7 possesses both ATPase and protease activities : the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments (By similarity). The m-AAA protease exerts a dual role in the mitochondrial inner membrane : it mediates the processing of specific regulatory proteins and ensures protein quality control by degrading misfolded polypeptides (By similarity). Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome (By similarity). Acts as a regulator of calcium in neurons by mediating degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (PubMed : 28396416, PubMed : 31097542). Also regulates mitochondrial calcium by catalyzing degradation of MCU (PubMed : 31097542). Plays a role in the formation and regulation of the mitochondrial permeability transition pore (mPTP) and its proteolytic activity is dispensable for this function (PubMed : 26387735).

See full target information SPG7

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPG7/PGN antibody [EPR26225-9]

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Western blot - Anti-SPG7/PGN antibody [EPR26225-9] (AB305255)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise