Rabbit Polyclonal Sphingomyelin Synthase 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGMS1 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SGMS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VZ5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
Target data
Function
Major sphingomyelin synthase at the Golgi apparatus (PubMed:14685263, PubMed:17449912). Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes (PubMed:14685263, PubMed:14976195, PubMed:17449912, PubMed:17982138, PubMed:19454763). Does not use free phosphorylcholine or CDP-choline as donor (PubMed:14685263, PubMed:14976195). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed:14976195, PubMed:17449912, PubMed:17982138). Plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (PubMed:18370930, PubMed:21980337).
Alternative names
MOB, SMS1, TMEM23, SGMS1, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Medulla oblongata-derived protein, Sphingomyelin synthase 1, Transmembrane protein 23, Protein Mob
Recommended products
Rabbit Polyclonal Sphingomyelin Synthase 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGMS1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SGMS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VZ5
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
>95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sphingomyelin Synthase 1 (SMS1) also known as sphingomyelin phosphatidylcholine transferase 1 is an enzyme that transfers a phosphatidylcholine group to ceramide forming sphingomyelin and diacylglycerol. It operates mainly in the Golgi apparatus and the plasma membrane. SMS1 has a molecular mass roughly 43 kDa. The enzyme exhibits strong expression in the liver kidney and brain indicating its importance in these tissues.
- Biological function summary
SMS1 plays an important role in sphingolipid metabolism by ensuring the balance between sphingomyelin and ceramide levels. The enzyme functions within membrane and lipid-associated processes potentially affecting cellular signaling and structure. SMS1 is not part of a well-defined complex but interacts with other sphingolipid metabolism proteins which supports cellular homeostasis.
- Pathways
SMS1 contributes significantly to the sphingolipid metabolic pathway and the ceramide biosynthesis pathway. These pathways are essential for maintaining cellular membranes and serve as substrates for complex lipid biosynthesis. SMS1 closely interacts with proteins like ceramide synthase and glucosylceramide synthase coordinating the flux through sphingolipid-related paths and influencing apoptotic and proliferative signals.
- Associated diseases and disorders
Disruptions in SMS1 function have links to atherosclerosis and neurodegenerative diseases. In atherosclerosis altered SMS1 activity can disrupt lipid balance encouraging lipid plaques in arteries. Furthermore SMS1 connections with proteins like acid sphingomyelinase may influence neurodegenerative conditions where sphingolipid imbalances contribute to cell death and dysfunction. These relationships highlight SMS1's emerging role in clinical research and as a possible therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Sphingomyelin Synthase 1 antibody (ab235057)
All lanes: Western blot - Anti-Sphingomyelin Synthase 1 antibody (ab235057) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 1 antibody (ab235057)
Paraffin-embedded human pancreatic tissue stained for Sphingomyelin Synthase 1 using ab235057 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
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