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AB235057

Anti-Sphingomyelin Synthase 1 antibody

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(2 Publications)

Rabbit Polyclonal Sphingomyelin Synthase 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SGMS1 aa 1-150.

View Alternative Names

MOB, SMS1, TMEM23, SGMS1, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Medulla oblongata-derived protein, Sphingomyelin synthase 1, Transmembrane protein 23, Protein Mob

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 1 antibody (AB235057)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 1 antibody (AB235057)

Paraffin-embedded human pancreatic tissue stained for Sphingomyelin Synthase 1 using ab235057 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

Western blot - Anti-Sphingomyelin Synthase 1 antibody (AB235057)
  • WB

Supplier Data

Western blot - Anti-Sphingomyelin Synthase 1 antibody (AB235057)

All lanes:

Western blot - Anti-Sphingomyelin Synthase 1 antibody (ab235057) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SGMS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q86VZ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sphingomyelin Synthase 1 (SMS1) also known as sphingomyelin phosphatidylcholine transferase 1 is an enzyme that transfers a phosphatidylcholine group to ceramide forming sphingomyelin and diacylglycerol. It operates mainly in the Golgi apparatus and the plasma membrane. SMS1 has a molecular mass roughly 43 kDa. The enzyme exhibits strong expression in the liver kidney and brain indicating its importance in these tissues.
Biological function summary

SMS1 plays an important role in sphingolipid metabolism by ensuring the balance between sphingomyelin and ceramide levels. The enzyme functions within membrane and lipid-associated processes potentially affecting cellular signaling and structure. SMS1 is not part of a well-defined complex but interacts with other sphingolipid metabolism proteins which supports cellular homeostasis.

Pathways

SMS1 contributes significantly to the sphingolipid metabolic pathway and the ceramide biosynthesis pathway. These pathways are essential for maintaining cellular membranes and serve as substrates for complex lipid biosynthesis. SMS1 closely interacts with proteins like ceramide synthase and glucosylceramide synthase coordinating the flux through sphingolipid-related paths and influencing apoptotic and proliferative signals.

Disruptions in SMS1 function have links to atherosclerosis and neurodegenerative diseases. In atherosclerosis altered SMS1 activity can disrupt lipid balance encouraging lipid plaques in arteries. Furthermore SMS1 connections with proteins like acid sphingomyelinase may influence neurodegenerative conditions where sphingolipid imbalances contribute to cell death and dysfunction. These relationships highlight SMS1's emerging role in clinical research and as a possible therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major sphingomyelin synthase at the Golgi apparatus (PubMed : 14685263, PubMed : 17449912). Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis : in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes (PubMed : 14685263, PubMed : 14976195, PubMed : 17449912, PubMed : 17982138, PubMed : 19454763). Does not use free phosphorylcholine or CDP-choline as donor (PubMed : 14685263, PubMed : 14976195). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed : 14976195, PubMed : 17449912, PubMed : 17982138). Plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (PubMed : 18370930, PubMed : 21980337).
See full target information SGMS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100559 PubMed38729351

2024

Sphingomyelin is involved in regulating UCP1-mediated nonshivering thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Detian Hu,Houyu Zhang,Zhen Liu,Carlos F Ibáñez,Cai Tie,Meng Xie

Journal of ovarian research 16:212 PubMed37940982

2023

Baicalein improves the chemoresistance of ovarian cancer through regulation of CirSLC7A6.

Applications

Unspecified application

Species

Unspecified reactive species

Shuqing Li,Zhihui Yi,Mingqing Li,Zhiling Zhu
View all publications
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