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AB302715

Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free)

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Rabbit Recombinant Monoclonal SPHK1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

SK1, SPHK, SPK, SPHK1, Sphingosine kinase 1, SK 1, SPK 1, Acetyltransferase SPHK1

4 Images
Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • WB

Supplier Data

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

This data was developed using ab302714, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 and 2 : 180 seconds Lane 3 : 15 seconds

All lanes:

Western blot - Anti-SPHK1 antibody [EPR24511-37] (<a href='/en-us/products/primary-antibodies/sphk1-antibody-epr24511-37-ab302714'>ab302714</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate 20 µg

Lane 2:

Human heart tissue lysate 20 µg

Lane 3:

human lu tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 42 kDa

false

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • WB

Supplier Data

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

This data was developed using ab302714, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 70 seconds

All lanes:

Western blot - Anti-SPHK1 antibody [EPR24511-37] (<a href='/en-us/products/primary-antibodies/sphk1-antibody-epr24511-37-ab302714'>ab302714</a>) at 1/1000 dilution

Lane 1:

U-118 MG (human brain glioma cell), whole cell lysate 20 µg

Lane 2:

SW1353 (human chondrosarcoma fibroblast), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 42 kDa

false

Exposure time: 70s

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • WB

Supplier Data

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

This data was developed using ab302714, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST The additional band at ~50 kDa is expected to be isoform 2 (Q9NYA1-2) of SPHK1 and its expression is also significantly reduced through SPHK1 knockdown. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SPHK1 antibody [EPR24511-37] (<a href='/en-us/products/primary-antibodies/sphk1-antibody-epr24511-37-ab302714'>ab302714</a>) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg

Lane 2:

Hela transfected with siRNA specifically targeti SPHK1, whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • WB

Supplier Data

Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

This data was developed using ab302714, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 70 seconds

All lanes:

Western blot - Anti-SPHK1 antibody [EPR24511-37] (<a href='/en-us/products/primary-antibodies/sphk1-antibody-epr24511-37-ab302714'>ab302714</a>) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 42 kDa

false

Exposure time: 70s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24511-37

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Western blot - Anti-SPHK2 (phospho T578) antibody (AB215750)

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Secondary Antibodies

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Reactivity data

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPHK1 also known as sphingosine kinase 1 is an enzyme that catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P). This target has a molecular mass of approximately 47 kDa. SPHK1 is expressed in various tissues including the brain spleen heart and lungs. Its activity plays a role in cell signaling processes that are essential for cellular responses to external stimuli.
Biological function summary

Within the cell SPHK1 participates in regulating cell survival proliferation and inflammation. It is part of the sphingolipid metabolic pathway which is not a traditional protein complex but more of a metabolic sequence. SPHK1 converts sphingosine a lipid signaling molecule into S1P which acts as a ligand for G-protein-coupled receptors. Through this conversion SPHK1 influences diverse cellular functions.

Pathways

This protein actively participates in the sphingolipid signaling pathway and the apoptosis pathway. In sphingolipid signaling S1P acts alongside ceramide and other sphingolipids to modulate cell response. SPHK1's interplay with the ceramide-sphingosine-S1P rheostat can determine cell fate by tipping the balance towards survival or apoptosis. In the apoptosis pathway SPHK1 serves as a regulator of cell death with its function connected to proteins such as Bcl-2 and Bax which are primary apoptosis regulators.

Abnormal expression of SPHK1 has links to cancer and cardiovascular diseases. In cancer overexpression of SPHK1 contributes to enhanced survival and proliferation of tumor cells with a noted interaction with the protein EGFR which is often upregulated in tumors. In cardiovascular diseases dysregulated SPHK1-S1P signaling can impact vascular integrity and inflammation influencing the progression of atherosclerosis with involvement of other inflammatory proteins like NF-kB.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed : 11923095, PubMed : 20577214, PubMed : 23602659, PubMed : 24929359, PubMed : 29662056). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed : 16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed : 20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed : 23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed : 24929359, PubMed : 28049734). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed : 25417698).. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
See full target information SPHK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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