Mouse Recombinant Monoclonal SPIB antibody. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (AB241570), expandable thumbnail](https://content.abcam.com/products/images/spib-antibody-235d-ab241570--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32001.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (AB241570)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (AB241570), expandable thumbnail](https://content.abcam.com/products/images/spib-antibody-235d-ab241570--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (AB241570)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 9.69 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
Alternative names
Transcription factor Spi-B, SPIB
Recommended products
Mouse Recombinant Monoclonal SPIB antibody. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 235D
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SPIB protein also known as Spi-B transcription factor is a member of the ETS family of transcription factors. Its molecular weight is approximately 31 kDa. SPIB is expressed mainly in lymphoid tissues including the spleen thymus and lymph nodes with high activity observed in B-cells and plasmacytoid dendritic cells. As a transcription factor SPIB binds to specific DNA sequences and influences the transcription of various genes involved in immune response and cellular development.
- Biological function summary
SPIB regulates gene expression important for immune system function. It plays a role in hematopoiesis particularly in the differentiation and function of B-cells and plasmacytoid dendritic cells. SPIB does not form complexes with other proteins in a classical sense but it does interact with other transcription factors within the cell’s nucleus to modulate gene expression. This regulation is significant for ensuring the immune system's proper response to pathogens.
- Pathways
SPIB influences several critical biological pathways. It is an important player in the Toll-like receptor (TLR) signaling pathway which is essential for recognizing pathogens and activating innate immunity. Within this pathway SPIB interacts with proteins such as IRF4 and IRF8 which also play roles in immune system regulation. SPIB's involvement extends to the B-cell receptor signaling pathway influencing the development and function of B-cells.
- Associated diseases and disorders
SPIB has associations with certain autoimmune conditions and hematological malignancies. In particular alterations in SPIB expression are linked to systemic lupus erythematosus (SLE) an autoimmune disease characterized by the immune system attacking its own tissues. Furthermore SPIB can influence the pathogenesis of some lymphomas such as diffuse large B-cell lymphoma (DLBCL) in which IRF4 also plays a pivotal role. Understanding SPIB's involvement in these diseases provides potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570), expandable thumbnail](https://content.abcam.com/products/images/spib-antibody-235d-ab241570--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32001.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling SPIB with ab241570 at 9.69μg/ml followed by a ready to use secondary. Nuclear staining on human spleen. The section was incubated with ab241570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570), expandable thumbnail](https://content.abcam.com/products/images/spib-antibody-235d-ab241570--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32986.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPIB antibody [235D] (ab241570)
Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling SPIB with ab241570 at 9.69μg/ml followed by a ready to use secondary. Nuclear staining on human thymoma. The section was incubated with ab241570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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