AB88467

Anti-SPIN90 antibody

Name: Anti-SPIN90 antibody (ab88467) | Abcam
Brand: Abcam Limited
SKU: AB88467
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse Polyclonal SPIN90 antibody. Carrier free. Suitable for WB and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCKIPSD.

View Alternative Names

AF3P21, SPIN90, NCKIPSD, NCK-interacting protein with SH3 domain, 54 kDa VacA-interacting protein, 54 kDa vimentin-interacting protein, 90 kDa SH3 protein interacting with Nck, AF3p21, Dia-interacting protein 1, Diaphanous protein-interacting protein, SH3 adapter protein SPIN90, WASP-interacting SH3-domain protein, Wiskott-Aldrich syndrome protein-interacting protein, VIP54, DIP-1, WISH

3 Images

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Western blot - Anti-SPIN90 antibody (AB88467)

All lanes:

Western blot - Anti-SPIN90 antibody (ab88467) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Unknown

Western blot - Anti-SPIN90 antibody (AB88467)

All lanes:

Western blot - Anti-SPIN90 antibody (ab88467) at 1 µg/mL

All lanes:

rat brain tissue lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Unknown

Western blot - Anti-SPIN90 antibody (AB88467)

All lanes:

Western blot - Anti-SPIN90 antibody (ab88467) at 1 µg/mL

Lane 1:

SPIN90 transfected 293T cell lysate at 50 µg

Lane 2:

Non transfected 293T cell lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NCKIPSD.

Q9NZQ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPIN90 also known as SH3 protein interacting with NCK with a molecular mass of approximately 90 kDa acts as a scaffold protein involved in the regulation of actin dynamics. SPIN90 belongs to a group of proteins known for their role in organizing protein complexes at actin filament sites. It facilitates the connection between the actin cytoskeleton and various signaling pathways. This protein shows expression mainly in the brain but is present in other tissues as well.

Biological function summary

Proteins such as SPIN90 help regulate the reorganization of the actin cytoskeleton important for cell movement and cytoskeletal integrity. It forms part of a larger complex that includes proteins like N-WASP and Arp2/3 complex. These interactions suggest that SPIN90 is an important player in actin polymerization and branching influencing cellular processes like migration endocytosis and morphogenesis.

Pathways

SPIN90 functions centrally in signaling pathways like the Rho GTPase signaling and epidermal growth factor receptor (EGFR) pathways. In these pathways SPIN90 interacts closely with proteins such as Cdc42 and Rac1 which are critical for controlling cell shape and promoting the formation of signal transduction complexes. This integration highlights the protein's influence in maintaining cell structure and promoting cell signaling responsiveness.

SPIN90 has associations with neurodegenerative disorders and cancer progression. Its interaction with proteins like Aβ and tau in the central nervous system suggests a role in Alzheimer's disease where cytoskeletal disruptions are a pathology hallmark. Furthermore SPIN90's relationship with proteins such as Src in cancer highlights its potential involvement in tumor cell motility and metastasis offering insights into mechanisms that drive these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.

See full target information NCKIPSD

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadf5143 PubMed37126564

2023

Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Nurul Izzati Wan Mohamad Noor,Nhung Thi Hong Nguyen,Theng Ho Cheong,Min Fey Chek,Toshio Hakoshima,Takehiko Inaba,Kyoko Hanawa-Suetsugu,Tamako Nishimura,Shiro Suetsugu

PloS one 7:e45085 PubMed23024796

2012

Dia-interacting protein (DIP) imposes migratory plasticity in mDia2-dependent tumor cells in three-dimensional matrices.

Applications

ICC/IF

Species

Human

Meghan M Wyse,Jun Lei,Andrea L Nestor-Kalinoski,Kathryn M Eisenmann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPIN90 antibody

Western blot - Anti-SPIN90 antibody (AB88467)

Western blot - Anti-SPIN90 antibody (AB88467)

Western blot - Anti-SPIN90 antibody (AB88467)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise