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AB196938

Anti-spindlin 1 antibody

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(2 Publications)

Rabbit Polyclonal spindlin 1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPIN1.

View Alternative Names

OCR, SPIN, SPIN1, Spindlin-1, Ovarian cancer-related protein, Spindlin1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-spindlin 1 antibody (AB196938)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-spindlin 1 antibody (AB196938)

Immunofluorescent analysis of A549 cells labeling spindlin 1 (green) with ab196938 at 1/50 dilution. Blue : DAPI for nuclear staining.

Western blot - Anti-spindlin 1 antibody (AB196938)
  • WB

Supplier Data

Western blot - Anti-spindlin 1 antibody (AB196938)

All lanes:

Western blot - Anti-spindlin 1 antibody (ab196938) at 1/500 dilution

Lane 1:

MCF7 cell extract

Lane 2:

SW480 cell extract

Lane 3:

293T cell extract

Lane 4:

Jurkat cell extract

Lane 5:

HeLa cell extract

Lane 6:

HepG2 cell extract

Lane 7:

SHSY5Y cell extract

Lane 8:

Mouse brain extract

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SPIN1.

Q9Y657

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spindlin 1 also known as SPIN1 is a protein that functions as a histone code reader. It weighs approximately 29 kDa and is highly expressed in the testes ovaries and certain cancer cell lines. Spindlin 1 has three tandem Tudor domains which allow it to interact with methylated histones specifically histone H3. These interactions enable SPIN1 to regulate gene expression by binding to modified histones and influencing the transcriptional machinery.
Biological function summary

SPIN1 acts as a regulator of gene expression by interacting with epigenetic markers on histones. It is part of the transcriptional regulation complex which includes other proteins involved in chromatin remodeling and gene activation. Its role in binding to trimethylated lysine 4 on histone H3 (H3K4me3) highlights its impact on transcriptional regulation processes. This interaction is important for maintaining the pluripotency of stem cells and possibly influencing cell differentiation.

Pathways

SPIN1 functions mainly within the epigenetic regulation and gene expression pathways. It affects the histone modification pathway and plays an important role in regulating the transcriptional activation process. SPIN1's relationship with other proteins like the histone methyltransferase SETD3 and components of the chromatin-remodeling complex defines its role in gene regulation mechanisms. Together these proteins determine chromatin structure and gene activity impacting transcriptional outcomes.

SPIN1 has associations with cancer particularly ovarian and testicular cancers. This link arises from its overexpression which can promote tumorigenesis by altering gene expression profiles in cancerous cells. SPIN1 also interacts with oncogenic pathways involving proteins like MYC further implicating it in cancer development. Additionally its abnormal functioning can contribute to infertility issues due to its vital role in reproductive tissues with links to proteins involved in spermatogenesis and oogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed : 24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed : 21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I : may act by regulating maternal transcripts to control meiotic resumption.
See full target information SPIN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:9113-9123 PubMed33061605

2020

Long Non-Coding RNA Regulates Expression to Accelerate Tumor Progression in Non-Small Cell Lung Cancer by Sponging .

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Chen,Pingan Song,Yuan Yao,Yang Yang

Cell metabolism 30:877-889.e7 PubMed31474565

2019

Glutamate Signaling in Hepatic Stellate Cells Drives Alcoholic Steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Won-Mook Choi,Hee-Hoon Kim,Myung-Ho Kim,Resat Cinar,Hyon-Seung Yi,Hyuk Soo Eun,Seok-Hwan Kim,Young Jae Choi,Young-Sun Lee,So Yeon Kim,Wonhyo Seo,Jun-Hee Lee,Young-Ri Shim,Ye Eun Kim,Keungmo Yang,Tom Ryu,Jung Hwan Hwang,Chul-Ho Lee,Hueng-Sik Choi,Bin Gao,Won Kim,Sang Kyum Kim,George Kunos,Won-Il Jeong
View all publications
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