AB203579

Anti-SPINK1/P12 antibody

Name: Anti-SPINK1/P12 antibody (ab203579) | Abcam
Brand: Abcam Limited
SKU: AB203579
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SPINK1/P12 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Serine protease inhibitor Kazal-type 1 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Serine protease inhibitor Kazal-type 1, Cholecystokinin-releasing peptide, Monitor peptide, Pancreatic secretory trypsin inhibitor, PSTI-I, Spink1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody (AB203579)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody (AB203579)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue sections labeling SPINK1/P12 with ab203579 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Rat Serine protease inhibitor Kazal-type 1 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P09655

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SPINK1/P12 protein also known as serine peptidase inhibitor Kazal type 1 has a mass of approximately 11 kDa. It functions as an inhibitor of serine proteases particularly trypsin. SPINK1 is mainly expressed in the pancreas but is also present in other tissues like the gastrointestinal tract and liver. In the pancreas SPINK1 plays an important role by preventing premature activation of digestive enzymes guarding pancreatic tissue from autodigestion.

Biological function summary

SPINK1 acts as a protector in the delicate balance of enzyme regulation in tissues where it is present. It does not form part of a larger protein complex but its action is critical in reducing excessive proteolytic activity. By inhibiting trypsin SPINK1 helps avoid damage to cells that could otherwise occur if protease activity goes unchecked. This inhibition supports the stability and regulated functioning of pancreatic and gastrointestinal tissues.

Pathways

SPINK1/P12 is involved in the protease inhibition pathway and plays a role in the regulation of digestive enzyme activity specifically linked to the pancreas. SPINK1 interacts with proteins such as trypsin and other serine proteases working to suppress their activity and protect against unwanted proteolysis. This interaction helps maintain the right balance within the digestive enzyme activation pathway ensuring proper digestive processes.

SPINK1 relates closely to pancreatic disorders like hereditary pancreatitis. Mutations or deficiencies in SPINK1 can lead to an increased risk of pancreatitis due to unchecked activation of trypsin and other proteases. It is also implicated in conditions such as chronic pancreatitis. SPINK1 mutations may also affect its interaction with trypsin exacerbating the tissue damage due to enzymatic digestion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed : 3202973, PubMed : 3597401). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).. In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production (By similarity).

See full target information Serine protease inhibitor Kazal-type 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Ophthalmology and therapy 10:63-74 PubMed33188486

2020

Expression of LIGHT/TNFSF14 and Its Receptors, HVEM and LTβR, Correlates with the Severity of Fibrosis in Lacrimal Sacs from Patients with Lacrimal Duct Obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Pawel Bielecki,Ewa Gindzienska-Sieskiewicz,Joanna Reszeć,Bartosz Piszczatowski,Marek Rogowski,Otylia Kowal-Bielecka,Krzysztof Kowal,Andrzej Sieskiewicz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPINK1/P12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody (AB203579)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise