AB207302

Anti-SPINK1/P12 antibody [EPR17585-116]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SPINK1/P12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PSTI, SPINK1, Serine protease inhibitor Kazal-type 1, Pancreatic secretory trypsin inhibitor, Tumor-associated trypsin inhibitor, TATI

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 minute; Lane 2 : 3 minutes.

SPINK1/P12 is a pancreatic secretory trypsin inhibitor, uniquely expressed in pancreas. Human fetal kidney lysate is used as negative control.

SPINK1/P12 contains a signal peptide, thus observed with a MW of 11 kDa (PMCID : PMC2765331)

All lanes:

Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302) at 1/5000 dilution

Lane 1:

Human pancreas lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 8 kDa

Observed band size: 11 kDa

false

Carrier free

Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17585-116

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SPINK1/P12 protein also known as serine peptidase inhibitor Kazal type 1 has a mass of approximately 11 kDa. It functions as an inhibitor of serine proteases particularly trypsin. SPINK1 is mainly expressed in the pancreas but is also present in other tissues like the gastrointestinal tract and liver. In the pancreas SPINK1 plays an important role by preventing premature activation of digestive enzymes guarding pancreatic tissue from autodigestion.

Biological function summary

SPINK1 acts as a protector in the delicate balance of enzyme regulation in tissues where it is present. It does not form part of a larger protein complex but its action is critical in reducing excessive proteolytic activity. By inhibiting trypsin SPINK1 helps avoid damage to cells that could otherwise occur if protease activity goes unchecked. This inhibition supports the stability and regulated functioning of pancreatic and gastrointestinal tissues.

Pathways

SPINK1/P12 is involved in the protease inhibition pathway and plays a role in the regulation of digestive enzyme activity specifically linked to the pancreas. SPINK1 interacts with proteins such as trypsin and other serine proteases working to suppress their activity and protect against unwanted proteolysis. This interaction helps maintain the right balance within the digestive enzyme activation pathway ensuring proper digestive processes.

SPINK1 relates closely to pancreatic disorders like hereditary pancreatitis. Mutations or deficiencies in SPINK1 can lead to an increased risk of pancreatitis due to unchecked activation of trypsin and other proteases. It is also implicated in conditions such as chronic pancreatitis. SPINK1 mutations may also affect its interaction with trypsin exacerbating the tissue damage due to enzymatic digestion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed : 7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).. In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.

See full target information SPINK1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Medicina (Kaunas, Lithuania) 61: PubMed40428778

2025

Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Cao,Yantong Wang,Dengsheng Xia

Medicina (Kaunas, Lithuania) 60: PubMed38256434

2024

PTEN, ERG, SPINK1, and TFF3 Status and Relationship in a Prostate Cancer Cohort from Jordanian Arab Population.

Applications

Unspecified application

Species

Unspecified reactive species

Samir Al Bashir,Mohammed S Alorjani,Khalid Kheirallah,Mohammad Al Hamad,Husam K Haddad,Ahmad Al-Dwairy,Baha A Bani-Fawwaz,Najla Aldaoud,Omar Halalsheh,Saddam Amawi,Ismail I Matalka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPINK1/P12 antibody [EPR17585-116]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (AB207302)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise