JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175929

Anti-SPINK4 antibody [EPR12492]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SPINK4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Serine protease inhibitor Kazal-type 4, Peptide PEC-60 homolog, SPINK4

1 Images
Western blot - Anti-SPINK4 antibody [EPR12492] (AB175929)
  • WB

Supplier Data

Western blot - Anti-SPINK4 antibody [EPR12492] (AB175929)

All lanes:

Western blot - Anti-SPINK4 antibody [EPR12492] (ab175929) at 1/1000 dilution

All lanes:

Small intestine lysate at 10 µg

Predicted band size: 9 kDa

false

  • Carrier free

    Anti-SPINK4 antibody [EPR12492] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12492

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB121257

Anti-SPINK4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK4 antibody (AB121257)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

  • 5
  • 7
Primary Antibodies

AB121202

Anti-FCGBP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGBP antibody (AB121202)

  • 0
  • 1
Primary Antibodies

AB255820

Anti-REG4 antibody [EPR22810-327]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPINK4 also known as Serine Peptidase Inhibitor Kazal-Type 4 functions as a protease inhibitor. It encodes a protein with a molecular mass of approximately 18 kDa. SPINK4 specifically inhibits certain serine proteases by forming stable complexes which prevents the proteases from degrading other proteins. It shows high expression in intestinal tissues particularly in the small intestine suggesting an important role in the digestive tract environment.
Biological function summary

SPINK4 modulates protease activity to maintain tissue homeostasis especially in the gut. This inhibitor regulates the activity of digestive enzymes ensuring controlled protein digestion and absorption. SPINK4 does not seem to be a part of a larger protein complex but acts individually to execute its inhibitory function. The precise control it gives helps maintain a balance between tissue protection and nutrient processing.

Pathways

SPINK4 engages in pathways related to protease inhibition in the gastrointestinal tract. It interacts closely with trypsin and other serine proteases to limit their proteolytic damage. SPINK4 is important in the protease-antiprotease pathway where it modulates enzyme activity to protect tissue from excessive protease action. This regulation is key in maintaining epithelial barrier integrity and efficient nutrient processing.

SPINK4 has potential links to inflammatory bowel disease (IBD) and colorectal cancer. Inflammatory conditions may result from imbalanced protease activity if SPINK4's regulatory function is disrupted. Conditions like IBD can see increased intestinal permeability where SPINK4 may be unable to adequately inhibit proteases contributing to tissue damage. Additionally correlations exist between SPINK4 expression and tumor progression possibly through interactions with cancer-related proteases like uPA (urokinase plasminogen activator) involved in tissue invasion.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information SPINK4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37998360

2023

Proximity Interactome Analysis of Super Conserved Receptors Expressed in the Brain Identifies EPB41L2, SLC3A2, and LRBA as Main Partners.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer Kaafarani,Romain Darche-Gabinaud,Xavier Bisteau,Virginie Imbault,Valérie Wittamer,Marc Parmentier,Isabelle Pirson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com