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AB249896

Anti-SPINK4 antibody [EPR12492] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SPINK4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Serine protease inhibitor Kazal-type 4, Peptide PEC-60 homolog, SPINK4

1 Images
Western blot - Anti-SPINK4 antibody [EPR12492] - BSA and Azide free (AB249896)
  • WB

Supplier Data

Western blot - Anti-SPINK4 antibody [EPR12492] - BSA and Azide free (AB249896)

This data was developed using ab175929, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SPINK4 antibody [EPR12492] (<a href='/en-us/products/primary-antibodies/spink4-antibody-epr12492-ab175929'>ab175929</a>) at 1/1000 dilution

All lanes:

Small intestine lysate at 10 µg

Predicted band size: 9 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12492

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249896 is the carrier-free version of ab175929.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPINK4 also known as Serine Peptidase Inhibitor Kazal-Type 4 functions as a protease inhibitor. It encodes a protein with a molecular mass of approximately 18 kDa. SPINK4 specifically inhibits certain serine proteases by forming stable complexes which prevents the proteases from degrading other proteins. It shows high expression in intestinal tissues particularly in the small intestine suggesting an important role in the digestive tract environment.
Biological function summary

SPINK4 modulates protease activity to maintain tissue homeostasis especially in the gut. This inhibitor regulates the activity of digestive enzymes ensuring controlled protein digestion and absorption. SPINK4 does not seem to be a part of a larger protein complex but acts individually to execute its inhibitory function. The precise control it gives helps maintain a balance between tissue protection and nutrient processing.

Pathways

SPINK4 engages in pathways related to protease inhibition in the gastrointestinal tract. It interacts closely with trypsin and other serine proteases to limit their proteolytic damage. SPINK4 is important in the protease-antiprotease pathway where it modulates enzyme activity to protect tissue from excessive protease action. This regulation is key in maintaining epithelial barrier integrity and efficient nutrient processing.

SPINK4 has potential links to inflammatory bowel disease (IBD) and colorectal cancer. Inflammatory conditions may result from imbalanced protease activity if SPINK4's regulatory function is disrupted. Conditions like IBD can see increased intestinal permeability where SPINK4 may be unable to adequately inhibit proteases contributing to tissue damage. Additionally correlations exist between SPINK4 expression and tumor progression possibly through interactions with cancer-related proteases like uPA (urokinase plasminogen activator) involved in tissue invasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SPINK4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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