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AB246898

Anti-SPINK5/LEKTI antibody

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(1 Publication)

Rabbit Polyclonal SPINK5/LEKTI antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPINK5 aa 450-600.

View Alternative Names

Serine protease inhibitor Kazal-type 5, Lympho-epithelial Kazal-type-related inhibitor, LEKTI, SPINK5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK5/LEKTI antibody (AB246898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK5/LEKTI antibody (AB246898)

Paraffin-embedded human tonsil tissue stained for SPINK5/LEKTI using ab246898 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK5/LEKTI antibody (AB246898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK5/LEKTI antibody (AB246898)

Paraffin-embedded human kidney tissue stained for SPINK5/LEKTI using ab246898 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SPINK5/LEKTI antibody (AB246898)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPINK5/LEKTI antibody (AB246898)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SPINK5/LEKTI (green) using ab246898 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SPINK5 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ38

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPINK5 also known as LEKTI (lympho-epithelial Kazal-type-related inhibitor) is a protease inhibitor with a molecular mass of approximately 130 kDa. This protein is predominantly expressed in epithelial tissues such as the skin thymus and mucosal surfaces like the lung and gut. SPINK5/LEKTI plays a mechanical role in inhibiting serine proteases which are critical for skin barrier functions. It regulates the activity of enzymes that are involved in the process of desquamation and maintaining skin integrity.
Biological function summary

SPINK5/LEKTI functions to maintain the balance of protease activity within skin tissue. It is part of a larger complex that regulates barrier homeostasis and influences desquamation by inhibiting enzymes such as kallikreins. This protease inhibition reduces excessive protease activity therefore preserving the epidermal barrier. By controlling the breakdown of structural components the protein ensures proper skin resilience and hydration retention.

Pathways

SPINK5/LEKTI is involved in the epidermal differentiation complex and interacts within the kallikrein-related pathway. It plays a role in regulating the kallikrein protease family including proteins such as KLK5 and KLK7 which are important for skin shedding and barrier formation. The inhibition of these proteases ensures a balanced turnover of skin cells preventing unwanted degradation of the skin's protective layer. SPINK5/LEKTI participates in pathways that balance cell renewal and contribute to protective mechanisms against environmental damage.

SPINK5/LEKTI is most notably linked to Netherton syndrome a genetic disorder characterized by compromised skin barrier function chronic inflammation and allergy susceptibility. Mutations in SPINK5 lead to uncontrolled protease activity due to reduced inhibition predominantly impacting related kallikrein activities. This connection extends to disorders of skin barrier failure. Additionally SPINK5/LEKTI may play a role in atopic dermatitis where aberrant protease activity contributes to the pathophysiology through similar mechanisms involving proteins like FLG (filaggrin) which affect skin hydration and barrier function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
See full target information SPINK5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of periodontal research 57:1183-1197 PubMed36146901

2022

Canonical and noncanonical pyroptosis are both activated in periodontal inflammation and bone resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Zhiwu Wu,Mingming Li,Jin Yang,Ran Cheng,Tao Hu
View all publications
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Product promise

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