AB138511

Anti-SPINK5/LEKTI antibody [EPR8777]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal SPINK5/LEKTI antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

Serine protease inhibitor Kazal-type 5, Lympho-epithelial Kazal-type-related inhibitor, LEKTI, SPINK5

2 Images

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Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

All lanes:

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/1000 dilution

Lane 1:

Fetal heart cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 121 kDa

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Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Lane 3:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 121 kDa

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Carrier free

Anti-SPINK5/LEKTI antibody [EPR8777] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8777

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPINK5 also known as LEKTI (lympho-epithelial Kazal-type-related inhibitor) is a protease inhibitor with a molecular mass of approximately 130 kDa. This protein is predominantly expressed in epithelial tissues such as the skin thymus and mucosal surfaces like the lung and gut. SPINK5/LEKTI plays a mechanical role in inhibiting serine proteases which are critical for skin barrier functions. It regulates the activity of enzymes that are involved in the process of desquamation and maintaining skin integrity.

Biological function summary

SPINK5/LEKTI functions to maintain the balance of protease activity within skin tissue. It is part of a larger complex that regulates barrier homeostasis and influences desquamation by inhibiting enzymes such as kallikreins. This protease inhibition reduces excessive protease activity therefore preserving the epidermal barrier. By controlling the breakdown of structural components the protein ensures proper skin resilience and hydration retention.

Pathways

SPINK5/LEKTI is involved in the epidermal differentiation complex and interacts within the kallikrein-related pathway. It plays a role in regulating the kallikrein protease family including proteins such as KLK5 and KLK7 which are important for skin shedding and barrier formation. The inhibition of these proteases ensures a balanced turnover of skin cells preventing unwanted degradation of the skin's protective layer. SPINK5/LEKTI participates in pathways that balance cell renewal and contribute to protective mechanisms against environmental damage.

SPINK5/LEKTI is most notably linked to Netherton syndrome a genetic disorder characterized by compromised skin barrier function chronic inflammation and allergy susceptibility. Mutations in SPINK5 lead to uncontrolled protease activity due to reduced inhibition predominantly impacting related kallikrein activities. This connection extends to disorders of skin barrier failure. Additionally SPINK5/LEKTI may play a role in atopic dermatitis where aberrant protease activity contributes to the pathophysiology through similar mechanisms involving proteins like FLG (filaggrin) which affect skin hydration and barrier function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.

See full target information SPINK5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica : PubMed40999902

2025

KDM4A regulates microglial polarization after ischemic stroke by regulating SPINK5 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Lei Xian,Ting Liu,Mengze Wang,Qing Zhao,Jiayi Hu,Rui Jing

Cellular & molecular biology letters 27:48 PubMed35705923

2022

Metformin inhibits melanoma cell metastasis by suppressing the miR-5100/SPINK5/STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Suwei,Xiao Yanbin,Wang Jianqiang,Ma Xiang,Peng Zhuohui,Kang Jianping,Wang Yunqing,Li Zhen

Experimental dermatology 31:223-232 PubMed34378233

2021

STAT3 maintains skin barrier integrity by modulating SPINK5 and KLK5 expression in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jaehyung Kim,Min-Gyu Kim,Sang Hoon Jeong,Hee Joo Kim,Sang Wook Son

Journal of leukocyte biology 103:799-805 PubMed29537705

2018

Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Alfredo Perales-Puchalt,Jairo Perez-Sanz,Kyle K Payne,Nikolaos Svoronos,Michael J Allegrezza,Ricardo A Chaurio,Carmen Anadon,Joseph Calmette,Subir Biswas,Jessica A Mine,Tara Lee Costich,Logan Nickels,Jayamanna Wickramasinghe,Melanie R Rutkowski,Jose R Conejo-Garcia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPINK5/LEKTI antibody [EPR8777]

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

Western blot - Anti-SPINK5/LEKTI antibody [EPR8777] (AB138511)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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