AB309135

Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Spinophilin/Neurabin 2 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.

View Alternative Names

PPP1R6, PPP1R9B, Neurabin-2, Neurabin-II, Protein phosphatase 1 regulatory subunit 9B, Spinophilin

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer (PMID : 25857299). The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebellum. The section was incubated with ab309135 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : no staining on human skeletal muscle. The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : almost no staining on human liver. The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebellum (PMID : 9275233). The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebellum (PMID : 9275233). The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/2000 (0.246 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Neurabin-2 (ab309135, green), anti-Map2 (ab225315, red) and anti-GFAP (ab201732, grey) on rat cerebellum. Panel B : anti-Neurabin-2 stained on rat cerebellum. Panel C : anti-Map2 stained in neurons of rat cerebellum. Panel D : anti-GFAP stained in astrocytes of rat cerebellum. The section was incubated in two rounds of staining : in the order of ab309135 and ab225315, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/2000 (0.246 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-Neurabin-2 (ab309135, green), anti-Map2 (ab225315, red) and anti-GFAP (ab201732, grey) on mouse cerebellum.Panel B : anti-Neurabin-2 stained on mouse cerebellum.Panel C : anti-Map2 stained in neurons of mouse cerebellum.Panel D : anti-GFAP stained in astrocytes of mouse cerebellum. The section was incubated in two rounds of staining : in the order of ab309135 and ab225315, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : no staining on mouse skeletal muscle (PMID : 9452470). The section was incubated with ab309135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/2000 (0.246 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression : confocal image showing no staining on mouse liver (PMID : 9452470). The nuclear counterstain was DAPI (Blue). The section was incubated with ab309135 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Spinophilin/Neurabin 2 with ab309135 at 1/2000 (0.246 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression : confocal image showing no staining on rat liver (PMID : 9452470). The nuclear counterstain was DAPI (Blue). The section was incubated with ab309135 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Carrier free

Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26265-105

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spinophilin also known as Neurabin 2 is a protein with a molecular weight of approximately 120 kDa. This protein is mostly found in the central nervous system where it associates with dendritic spines of neurons. Spinophilin contains an actin-binding domain a PDZ domain and a coiled-coil domain which allows it to interact with various proteins involved in synaptic function. Its distribution is not limited to the brain as it has also been identified in other tissues like the liver and kidneys.

Biological function summary

Spinophilin functions in the regulation of synaptic signaling and structural plasticity contributing to neural communication. It often forms part of signaling complexes binding to protein phosphatase 1 (PP1) to modulate dephosphorylation activities. Spinophilin influences the organization and dynamics of actin filaments affecting the stability and morphology of dendritic spines which are critical sites for synaptic transmission and plasticity. Through these interactions Spinophilin plays an important role in learning and memory processes.

Pathways

Spinophilin interacts with systems involved in synaptic signaling and cytoskeletal organization. It impacts the RhoA/ROCK pathway which is essential for actin filament dynamics by modulating PP1 activity. Additionally Spinophilin indirectly affects the mTOR pathway which is involved in protein synthesis and cell growth. It relates to proteins such as RhoA and mTOR through its role in maintaining the balance of phosphorylation states within the cell.

Spinophilin's association with synaptic signaling links it to neurodegenerative diseases such as Alzheimer's disease where synaptic dysfunction is a characteristic feature. Furthermore Spinophilin's involvement in spine morphology suggests a role in psychiatric disorders like schizophrenia where altered spine density and structure are observed. In these diseases Spinophilin connects with proteins like tau in Alzheimer's and dopamine receptors in schizophrenia highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.

See full target information PPP1R9B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Immunohistochemistry (Frozen sections) - Anti-Spinophilin/Neurabin 2 antibody [EPR26265-105] (AB309135)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise