AB130403

Anti-Spir-1 antibody

Name: Anti-Spir-1 antibody (ab130403) | Abcam
Brand: Abcam Limited
SKU: AB130403

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(1 Publication)

Rabbit Polyclonal Spir-1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPIRE1 aa 350 to C-terminus.

View Alternative Names

KIAA1135, SPIR1, SPIRE1, Protein spire homolog 1, Spir-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spir-1 antibody (AB130403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spir-1 antibody (AB130403)

Immunohistochemical analysis of paraffin embedded human lateral ventricle tissue labeling Spir-1 in the nucleus of neuronal cells with ab130403 antibody at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Spir-1 antibody (AB130403)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Spir-1 antibody (AB130403)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling Spir-1 in the nucleoplasm and cytosol with ab130403 antibody at 2 μg/ml.

Supplier Data

Western blot - Anti-Spir-1 antibody (AB130403)

All lanes:

Western blot - Anti-Spir-1 antibody (ab130403) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPIRE1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08AE8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" } } }

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Product details

Store product undiluted. For continuous use, store at 2-8°C for one-two days. Working dilution samples should be discarded if not used within 12 hours. The antibody solution should be gently mixed before use.

This antibody is mono-specific.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spir-1 also known as SPIR1 is a protein that functions as an actin nucleator. Its molecular mass is approximately 82 kDa. Spir-1 polymerizes actin by initiating the formation of new actin filaments. It has a role in controlling cytoskeletal dynamics important for cellular structure and movement. Spir-1 is expressed in a variety of human tissues including the brain where it shows particular activity in embryonic and neural tissues. These actin-modulating properties contribute to its regulation of cellular processes fundamental for organismal development.

Biological function summary

Spir-1 influences the organization of the actin cytoskeleton acting as an important factor in cellular motility and shape determination. It forms a complex with other actin-related proteins such as the formin protein family. This complex enables Spir-1 to work efficiently in actin polymerization ensuring the proper assembly of actin filaments. Spir-1's role becomes critical during processes like cell division and intracellular transport where actin dynamics are essential.

Pathways

Spir-1 participates in the regulation of the actin cytoskeleton pathway integral for maintaining cellular architecture and facilitating intracellular transport. Spir-1 closely interacts with proteins like formin homology proteins to control actin dynamics within this pathway. Another important pathway where Spir-1 is active is the signal transduction pathway which involves communication within cells to coordinate responses to external signals influencing how cells change their behavior or state in reaction to environmental cues.

Spir-1 has associations with neurodevelopmental disorders and cancer. Disruptions in actin dynamics influenced by aberrant Spir-1 function may lead to impaired neural development linking it to developmental brain disorders. Additionally anomalous Spir-1 activity could contribute to cancer progression as changes in cytoskeleton organization can affect cell proliferation and movement elements important for tumor growth and metastasis. Proteins such as cortactin another actin-associated protein also tie into these disease processes highlighting the complex network of actin regulatory proteins in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed : 11747823, PubMed : 21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed : 11747823). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed : 21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed : 21620703). Also acts in the nucleus : together with FMN2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed : 26287480). In addition, promotes innate immune signaling downstream of dsRNA sensing (PubMed : 35148361). Mechanistically, contributes to IRF3 phosphorylation and activation downstream of MAVS and upstream of TBK1 (PubMed : 35148361).

See full target information SPIRE1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 12:7437-7450 PubMed31686849

2019

Ultrasound-microbubbles-mediated microRNA-449a inhibits lung cancer cell growth via the regulation of Notch1.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwu Meng,Shaofei Yuan,Linjia Zhu,Zongxiao ShangGuan,Renguo Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Spir-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spir-1 antibody (AB130403)

Immunocytochemistry/ Immunofluorescence - Anti-Spir-1 antibody (AB130403)

Western blot - Anti-Spir-1 antibody (AB130403)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise