Rabbit Polyclonal sPLA2-IIE antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2G2E aa 1-50.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | |
---|---|
Human | Tested |
Mouse | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Select an associated product type
Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10681567, PubMed:11922621, PubMed:28883454). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), releasing various unsaturated fatty acids including oleoate, linoleoate, arachidonate, docosahexaenoate and lysophosphatidylethanolamines in preference to lysophosphatidylcholines (PubMed:10681567, PubMed:28883454). In response to high-fat diet, hydrolyzes minor lipoprotein phospholipids including phosphatidylserines, phosphatidylinositols and phosphatidylglycerols, altering lipoprotein composition and fat storage in adipose tissue and liver (By similarity). May act in an autocrine and paracrine manner (PubMed:11922621). Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria (PubMed:11922621). Acts as a hair follicle phospholipase A2. Selectively releases lysophosphatidylethanolamines (LPE) and various unsaturated fatty acids in skin to regulate hair follicle homeostasis (By similarity). May regulate the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes (PubMed:11922621). Upon allergen exposure, may participate in allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells (By similarity).
Group IIE secretory phospholipase A2, GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E, PLA2G2E
Rabbit Polyclonal sPLA2-IIE antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2G2E aa 1-50.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Secretory phospholipase A2 group IIE (sPLA2-IIE) is an enzyme belonging to the phospholipase A2 family also known as PLA2G2E. It has a molecular mass of approximately 14 kDa. sPLA2-IIE hydrolyzes the sn-2 position of glycerophospholipids releasing free fatty acids and lysophospholipids. This enzyme shows expression in various tissues including the pancreas and inflammatory sites where it contributes to lipid metabolism and cell membrane remodeling.
Secretory phospholipase A2 group IIE plays a role in inflammation and immune responses. Its activity results in the production of arachidonic acid and lysophospholipids which are precursors for eicosanoids like prostaglandins and leukotrienes. These lipid mediators regulate inflammatory processes thereby altering cellular responses. sPLA2-IIE participates in a complex network of enzymes and receptors modulating lipid signaling and affecting diverse physiological functions.
SPLA2-IIE interacts within the arachidonic acid pathway and the eicosanoid biosynthesis pathway. In these pathways sPLA2-IIE generates bioactive lipids impacting inflammatory signaling. It functions alongside proteins such as cyclooxygenase (COX) and lipoxygenase (LOX) which further metabolize arachidonic acid into specific eicosanoids. These pathways illustrate the enzyme's contribution to finely tuning the inflammatory response and cellular signaling networks.
Secretory phospholipase A2 group IIE associates with conditions like rheumatoid arthritis and atherosclerosis. Its enzymatic activity correlates with the inflammatory components of these diseases promoting tissue damage and disease progression. Through its involvement in lipid mediator synthesis sPLA2-IIE interacts with proteins like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) key players in the inflammatory pathways that drive these disorders. Understanding the role of sPLA2-IIE in these diseases can help develop targeted therapies.
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Immunohistochemical staining of Human heart muscle tissue labeling sPLA2-IIE using ab204576 at 1/20 diluiton.
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