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Rabbit Polyclonal sPLA2-IIE antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2G2E aa 1-50.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-IIE antibody (AB204576), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PLA2G2E aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZK7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10681567, PubMed:11922621, PubMed:28883454). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), releasing various unsaturated fatty acids including oleoate, linoleoate, arachidonate, docosahexaenoate and lysophosphatidylethanolamines in preference to lysophosphatidylcholines (PubMed:10681567, PubMed:28883454). In response to high-fat diet, hydrolyzes minor lipoprotein phospholipids including phosphatidylserines, phosphatidylinositols and phosphatidylglycerols, altering lipoprotein composition and fat storage in adipose tissue and liver (By similarity). May act in an autocrine and paracrine manner (PubMed:11922621). Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria (PubMed:11922621). Acts as a hair follicle phospholipase A2. Selectively releases lysophosphatidylethanolamines (LPE) and various unsaturated fatty acids in skin to regulate hair follicle homeostasis (By similarity). May regulate the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes (PubMed:11922621). Upon allergen exposure, may participate in allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal sPLA2-IIE antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2G2E aa 1-50.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PLA2G2E aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZK7
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Secretory phospholipase A2 group IIE (sPLA2-IIE) is an enzyme belonging to the phospholipase A2 family also known as PLA2G2E. It has a molecular mass of approximately 14 kDa. sPLA2-IIE hydrolyzes the sn-2 position of glycerophospholipids releasing free fatty acids and lysophospholipids. This enzyme shows expression in various tissues including the pancreas and inflammatory sites where it contributes to lipid metabolism and cell membrane remodeling.

Biological function summary

Secretory phospholipase A2 group IIE plays a role in inflammation and immune responses. Its activity results in the production of arachidonic acid and lysophospholipids which are precursors for eicosanoids like prostaglandins and leukotrienes. These lipid mediators regulate inflammatory processes thereby altering cellular responses. sPLA2-IIE participates in a complex network of enzymes and receptors modulating lipid signaling and affecting diverse physiological functions.

Pathways

SPLA2-IIE interacts within the arachidonic acid pathway and the eicosanoid biosynthesis pathway. In these pathways sPLA2-IIE generates bioactive lipids impacting inflammatory signaling. It functions alongside proteins such as cyclooxygenase (COX) and lipoxygenase (LOX) which further metabolize arachidonic acid into specific eicosanoids. These pathways illustrate the enzyme's contribution to finely tuning the inflammatory response and cellular signaling networks.

Associated diseases and disorders

Secretory phospholipase A2 group IIE associates with conditions like rheumatoid arthritis and atherosclerosis. Its enzymatic activity correlates with the inflammatory components of these diseases promoting tissue damage and disease progression. Through its involvement in lipid mediator synthesis sPLA2-IIE interacts with proteins like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) key players in the inflammatory pathways that drive these disorders. Understanding the role of sPLA2-IIE in these diseases can help develop targeted therapies.

Product promise

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-IIE antibody (ab204576), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-IIE antibody (ab204576)

    Immunohistochemical staining of Human heart muscle tissue labeling sPLA2-IIE using ab204576 at 1/20 diluiton.

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Product protocols

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