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AB189274

Anti-splicing factor 1 antibody

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(1 Publication)

Rabbit Polyclonal splicing factor 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SF1 aa 1-100.

View Alternative Names

ZFM1, ZNF162, SF1, Splicing factor 1, Mammalian branch point-binding protein, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, mBBP

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody (AB189274)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody (AB189274)

Immunofluorescence analysis of HeLa cells, labeling splicing factor 1 with ab189274 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody (AB189274)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody (AB189274)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue, labeling splicing factor 1 with ab189274 at 10 μg/ml.

Western blot - Anti-splicing factor 1 antibody (AB189274)
  • WB

Supplier Data

Western blot - Anti-splicing factor 1 antibody (AB189274)

All lanes:

Western blot - Anti-splicing factor 1 antibody (ab189274) at 1/500 dilution

Lane 1:

COLO205 cell extract

Lane 2:

COLO205 cell extract with synthesized peptide

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SF1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q15637

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Splicing factor 1 also known as SF1 or mBBP plays an important role in the splicing of pre-mRNA. It binds to the branch point sequence of intronic regions facilitating the assembly of the spliceosome complex necessary for excising introns and joining exons. The protein has a molecular mass of approximately 75 kDa. SF1 is expressed in a variety of tissues indicating its fundamental role in mRNA processing across different cell types.
Biological function summary

The protein engages in the early stages of spliceosome complex formation working in concert with other splicing factors such as U2AF and SF3b. SF1 is a specific component required in spliceosome assembly contributing to the recognition of correct splice sites. This step is essential for ensuring the accuracy and efficiency of mRNA splicing which is critical for generating mature mRNA transcripts ready for translation. SF1’s involvement with the spliceosome complex itself highlights its importance in cell function regulation.

Pathways

SF1's activity influences the splicing pathway. This pathway a part of the comprehensive gene expression mechanism is essential for regulating cellular processes through mRNA modification. SF1 interacts with proteins like U2AF65 and U2 snRNP vital components of the spliceosome to facilitate splicing. Additionally SF1 indirectly impacts alternative splicing pathways thereby contributing to mRNA diversity and functionality.

Aberrant SF1 function relates to cancer and neurological disorders. Alterations in SF1 expression or mutations can disrupt normal splicing leading to dysregulation of gene expression and pathogenic outcomes. In particular SF1 has been implicated in breast cancer and spinal muscular atrophy. It connects to other proteins such as BRCA1 in cancer and SMN1 in spinal muscular atrophy highlighting how its dysregulation can contribute to disease pathogenesis. Understanding SF1's function and interactions could enhance knowledge of disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
See full target information SF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 562:133-139 PubMed30250253

2018

Discovery of a periosteal stem cell mediating intramembranous bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Shawon Debnath,Alisha R Yallowitz,Jason McCormick,Sarfaraz Lalani,Tuo Zhang,Ren Xu,Na Li,Yifang Liu,Yeon Suk Yang,Mark Eiseman,Jae-Hyuck Shim,Meera Hameed,John H Healey,Mathias P Bostrom,Dan Avi Landau,Matthew B Greenblatt
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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