Rabbit Polyclonal SPNS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPNS1.
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Publications
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- Nature immunology 22:423-4332021Multi-omics analyses reveal that HIV-1 alters CD4 T cell immunometabolism to fuel virus replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitao Guo,Qi Wang,Khader Ghneim,Li Wang,Elena Rampanelli,Elizabeth Holley-Guthrie,Liang Cheng,Carolina Garrido,David M Margolis,Leigh A Eller,Merlin L Robb,Rafick-Pierre Sekaly,Xian Chen,Lishan Su,Jenny P-Y TingPubMed 33767427
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SPNS1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2V7
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Plays a critical role in the phospholipid salvage pathway from lysosomes to the cytosol (PubMed:36161949, PubMed:37075117). Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids, which can then be reacylated by acyltransferases in the endoplasmic reticulum to form phospholipids (PubMed:36161949, PubMed:37075117). Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), can also transport lysophosphatidylglycerol (LPG), but not other anionic lysophospholipids, sphingosine, nor sphingomyelin (PubMed:36161949). Transports lysophospholipids with saturated, monounsaturated, and polyunsaturated fatty acids, such as 1-hexadecanoyl-sn-glycero-3-phosphocholine, 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine and 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine, respectively (PubMed:36161949, PubMed:37075117). Can also transport lysoplasmalogen (LPC with a fatty alcohol) such as 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine (PubMed:36161949). Lysosomal LPC could function as intracellular signaling messenger (PubMed:37075117). Essential player in lysosomal homeostasis (PubMed:36161949). Crucial for cell survival under conditions of nutrient limitation (PubMed:37075117). May be involved in necrotic or autophagic cell death (PubMed:12815463).
Alternative names
SPIN1, PP20300, SPNS1, Protein spinster homolog 1, HSpin1, Spinster-like protein 1
Recommended products
Rabbit Polyclonal SPNS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPNS1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SPNS1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2V7
- Purity
- Whole antiserum
- Specificity
This antibody reacts specifically with human 56 kDa SPNS1 protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPNS1 also known as Spinster homolog 1 is a transporter protein with a molecular weight of approximately 39 kDa. This protein is mainly found in tissues such as placenta brain lung and kidney. Mechanically SPNS1 functions as a transporter that facilitates the movement of sphingosine-1-phosphate (S1P) across the cellular membranes. This transport activity plays an essential role in regulating S1P levels within and outside cells impacting various cellular processes.
- Biological function summary
SPNS1 serves as a pivotal regulator in lipid signaling and cell migration. It is part of a system that balances the synthesis and degradation of S1P influencing numerous cellular functions including immune cell trafficking and vascular development. SPNS1’s activity is integral to maintaining the cellular gradient of S1P necessary for directing cellular responses in physiological contexts like immune response and cellular development. Its function links to several cellular outcomes making it a significant player in cellular communication.
- Pathways
SPNS1 significantly contributes to the sphingolipid signaling pathway and the vascular endothelial growth factor (VEGF) pathway. Within the sphingolipid signaling pathway SPNS1 works closely with sphingosine kinases which phosphorylate sphingosine to S1P and S1P receptors that mediate downstream signaling. In the VEGF pathway SPNS1 indirectly influences angiogenesis through lipid signaling mediators. These pathways highlight the interactions of SPNS1 with other pivotal proteins like sphingosine kinases and S1P receptors.
- Associated diseases and disorders
SPNS1 holds relevance to conditions like cancer and autoimmune disorders. Its role in sphingolipid signaling associates it with cancer progression where altered S1P levels can affect tumor growth and metastasis. In autoimmune disorders dysregulated S1P signaling potentially involving SPNS1 can influence immune cell migration and function aggravating disease symptoms. Proteins like sphingosine kinases and S1P receptors are also involved in these diseases linking further to SPNS1's impact on pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SPNS1 antibody (ab59971)
All lanes: Western blot - Anti-SPNS1 antibody (ab59971) at 1/1000 dilution
Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 56 kDa
Observed band size: 49 kDa, 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (ab59971)
ICC/IF image of ab59971 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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