AB59971

Anti-SPNS1 antibody

Name: Anti-SPNS1 antibody (ab59971) | Abcam
Brand: Abcam Limited
SKU: AB59971
Price: 525 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(2 Publications)

Rabbit Polyclonal SPNS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SPNS1.

View Alternative Names

SPIN1, PP20300, SPNS1, Protein spinster homolog 1, HSpin1, Spinster-like protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (AB59971)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (AB59971)

ICC/IF image of ab59971 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-SPNS1 antibody (AB59971)

All lanes:

Western blot - Anti-SPNS1 antibody (ab59971) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 56 kDa

Observed band size: 49 kDa,56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SPNS1. The exact immunogen used to generate this antibody is proprietary information.

Q9H2V7

Specificity

This antibody reacts specifically with human 56 kDa SPNS1 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPNS1 also known as Spinster homolog 1 is a transporter protein with a molecular weight of approximately 39 kDa. This protein is mainly found in tissues such as placenta brain lung and kidney. Mechanically SPNS1 functions as a transporter that facilitates the movement of sphingosine-1-phosphate (S1P) across the cellular membranes. This transport activity plays an essential role in regulating S1P levels within and outside cells impacting various cellular processes.

Biological function summary

SPNS1 serves as a pivotal regulator in lipid signaling and cell migration. It is part of a system that balances the synthesis and degradation of S1P influencing numerous cellular functions including immune cell trafficking and vascular development. SPNS1’s activity is integral to maintaining the cellular gradient of S1P necessary for directing cellular responses in physiological contexts like immune response and cellular development. Its function links to several cellular outcomes making it a significant player in cellular communication.

Pathways

SPNS1 significantly contributes to the sphingolipid signaling pathway and the vascular endothelial growth factor (VEGF) pathway. Within the sphingolipid signaling pathway SPNS1 works closely with sphingosine kinases which phosphorylate sphingosine to S1P and S1P receptors that mediate downstream signaling. In the VEGF pathway SPNS1 indirectly influences angiogenesis through lipid signaling mediators. These pathways highlight the interactions of SPNS1 with other pivotal proteins like sphingosine kinases and S1P receptors.

SPNS1 holds relevance to conditions like cancer and autoimmune disorders. Its role in sphingolipid signaling associates it with cancer progression where altered S1P levels can affect tumor growth and metastasis. In autoimmune disorders dysregulated S1P signaling potentially involving SPNS1 can influence immune cell migration and function aggravating disease symptoms. Proteins like sphingosine kinases and S1P receptors are also involved in these diseases linking further to SPNS1's impact on pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a critical role in the phospholipid salvage pathway from lysosomes to the cytosol (PubMed : 36161949, PubMed : 37075117). Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids, which can then be reacylated by acyltransferases in the endoplasmic reticulum to form phospholipids (PubMed : 36161949, PubMed : 37075117). Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), can also transport lysophosphatidylglycerol (LPG), but not other anionic lysophospholipids, sphingosine, nor sphingomyelin (PubMed : 36161949). Transports lysophospholipids with saturated, monounsaturated, and polyunsaturated fatty acids, such as 1-hexadecanoyl-sn-glycero-3-phosphocholine, 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine and 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine, respectively (PubMed : 36161949, PubMed : 37075117). Can also transport lysoplasmalogen (LPC with a fatty alcohol) such as 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine (PubMed : 36161949). Lysosomal LPC could function as intracellular signaling messenger (PubMed : 37075117). Essential player in lysosomal homeostasis (PubMed : 36161949). Crucial for cell survival under conditions of nutrient limitation (PubMed : 37075117). May be involved in necrotic or autophagic cell death (PubMed : 12815463).

See full target information SPNS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 22:423-433 PubMed33767427

2021

Multi-omics analyses reveal that HIV-1 alters CD4 T cell immunometabolism to fuel virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Guo,Qi Wang,Khader Ghneim,Li Wang,Elena Rampanelli,Elizabeth Holley-Guthrie,Liang Cheng,Carolina Garrido,David M Margolis,Leigh A Eller,Merlin L Robb,Rafick-Pierre Sekaly,Xian Chen,Lishan Su,Jenny P-Y Ting

Frontiers in medicine 7:155 PubMed32411715

2020

G-CSF Infusion for Stem Cell Mobilization Transiently Increases Serum Cell-Free DNA and Protease Concentrations.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Stoikou,Shane V van Breda,Günther Schäfer,Lenka Vokalova,Stavros Giaglis,Alexandra Plattner,Laura Infanti,Andreas Holbro,Sinuhe Hahn,Simona W Rossi,Andreas Buser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPNS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (AB59971)

Western blot - Anti-SPNS1 antibody (AB59971)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise