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AB224071

Anti-SPOCK1 antibody

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(1 Publication)

Rabbit Polyclonal SPOCK1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPOCK1 aa 250-400.

View Alternative Names

SPOCK, TIC1, TICN1, SPOCK1, Testican-1, Protein SPOCK

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (AB224071)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (AB224071)

Paraffin-embedded human prostate tissue stained for SPOCK1 using ab224071 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPOCK1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q08629

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPOCK1 also known as Testican-1 is a proteoglycan involved mechanically in the regulation of cell-matrix interactions. It's a protein of approximately 54 kDa mass. SPOCK1 is expressed in various tissues with significant expression in the brain and skeletal muscle. In the extracellular matrix it forms part of the calcium-binding protein family having an active role in modulating matrix metalloproteinases (MMPs) and serine protease activities.
Biological function summary

SPOCK1 influences cellular adhesion migration and communication. It interacts with several extracellular matrix components participating actively in the structural organization of tissues. SPOCK1 often associates with certain complexes binding to proteins like fibronectin and laminin which are important for tissue integrity. Its function can impact cell signaling and tissue remodeling reflecting its diverse biological roles.

Pathways

SPOCK1 plays significant roles in the Wnt signaling pathway and the tissue inhibitors of metalloproteinases (TIMPs) pathway. In these pathways SPOCK1 modulates activities of proteins such as MMP2 and MMP9 which are pivotal for extracellular matrix degradation and remodeling. Its interaction with Wnt proteins highlights SPOCK1's influence in developmental and disease processes requiring precise cellular communication.

SPOCK1 links to cancers and fibrotic diseases. In cancer its overexpression often correlates with tumor progression and metastasis interacting with proteins like integrins to facilitate these processes. In fibrosis altered SPOCK1 levels affect extracellular matrix composition and deposition in conjunction with proteins like collagen contributing to tissue scarring and dysfunction. Understanding SPOCK1's roles provides insight into these conditions' pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.
See full target information SPOCK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0213751 PubMed30856233

2019

Development of a high-throughput fluorescent no-wash sodium influx assay.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan Tay,Teneale A Stewart,Felicity M Davis,Jennifer R Deuis,Irina Vetter
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Product promise

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