AB229935

Anti-SPOCK1 antibody

Name: Anti-SPOCK1 antibody (ab229935) | Abcam
Brand: Abcam Limited
SKU: AB229935
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(15 Publications)

Rabbit Polyclonal SPOCK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOCK1 aa 250 to C-terminus.

View Alternative Names

SPOCK, TIC1, TICN1, SPOCK1, Testican-1, Protein SPOCK

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (AB229935)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (AB229935)

Paraffin-embedded human kidney tissue stained for SPOCK1 using ab229935 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SPOCK1 antibody (AB229935)

Lane 1 : 293T whole cell lysate.

ab229935 antibody at 1 : 2000 dilution.

Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.

Predicted band size : 50 kDa Observed band size : 50 kDa.

All lanes:

Western blot - Anti-SPOCK1 antibody (ab229935)

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPOCK1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08629

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPOCK1 also known as Testican-1 is a proteoglycan involved mechanically in the regulation of cell-matrix interactions. It's a protein of approximately 54 kDa mass. SPOCK1 is expressed in various tissues with significant expression in the brain and skeletal muscle. In the extracellular matrix it forms part of the calcium-binding protein family having an active role in modulating matrix metalloproteinases (MMPs) and serine protease activities.

Biological function summary

SPOCK1 influences cellular adhesion migration and communication. It interacts with several extracellular matrix components participating actively in the structural organization of tissues. SPOCK1 often associates with certain complexes binding to proteins like fibronectin and laminin which are important for tissue integrity. Its function can impact cell signaling and tissue remodeling reflecting its diverse biological roles.

Pathways

SPOCK1 plays significant roles in the Wnt signaling pathway and the tissue inhibitors of metalloproteinases (TIMPs) pathway. In these pathways SPOCK1 modulates activities of proteins such as MMP2 and MMP9 which are pivotal for extracellular matrix degradation and remodeling. Its interaction with Wnt proteins highlights SPOCK1's influence in developmental and disease processes requiring precise cellular communication.

SPOCK1 links to cancers and fibrotic diseases. In cancer its overexpression often correlates with tumor progression and metastasis interacting with proteins like integrins to facilitate these processes. In fibrosis altered SPOCK1 levels affect extracellular matrix composition and deposition in conjunction with proteins like collagen contributing to tissue scarring and dysfunction. Understanding SPOCK1's roles provides insight into these conditions' pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

See full target information SPOCK1

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1619171 PubMed40837013

2025

SPOCK1 promotes the progression of breast cancer by modulating cancer-associated fibroblasts and exerts a synergistic effect with ANXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Jie,Xin Fei,Meng Fan

The Journal of clinical investigation 133: PubMed36881486

2023

Morphology-guided transcriptomic analysis of human pancreatic cancer organoids reveals microenvironmental signals that enhance invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yea Ji Jeong,Hildur Knutsdottir,Fatemeh Shojaeian,Michael G Lerner,Maria F Wissler,Elodie Henriet,Tammy Ng,Shalini Datta,Bernat Navarro-Serer,Peter Chianchiano,Benedict Kinny-Köster,Jacquelyn W Zimmerman,Genevieve Stein-O'Brien,Matthias M Gaida,James R Eshleman,Ming-Tseh Lin,Elana J Fertig,Andrew J Ewald,Joel S Bader,Laura D Wood

BMC gastroenterology 23:4 PubMed36611136

2023

SPOCK1 and POSTN are valuable prognostic biomarkers and correlate with tumor immune infiltrates in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Caiqin Gan,Mengting Li,Yuanyuan Lu,Ganjing Peng,Wenjie Li,Haizhou Wang,Yanan Peng,Qian Hu,Wanhui Wei,Fan Wang,Lan Liu,Qiu Zhao

Heliyon 8:e11321 PubMed36406681

2022

Identification by genetic algorithm optimized back propagation artificial neural network and validation of a four-gene signature for diagnosis and prognosis of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchong Li,Zuyi Ma,Qi Zhou,Shujie Wang,Qian Yan,Hongkai Zhuang,Zixuan Zhou,Chunsheng Liu,Zhongshi Wu,Jinglin Zhao,Shanzhou Huang,Chuanzhao Zhang,Baohua Hou

Cell cycle (Georgetown, Tex.) 21:2192-2205 PubMed35775884

2022

Circ_0061395 functions as an oncogenic gene in hepatocellular carcinoma by acting as a miR-1182 sponge.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Wu,Zhenhua Zhou,Chao Chen,Ming Chen

Pathology oncology research : POR 28:1610293 PubMed35221802

2022

Expression and Potential Prognostic Value of SOX9, MCL-1 and SPOCK1 in Gastric Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Luo,Teddy S Nagaria,Hongxia Sun,Junsheng Ma,Jamie L Lombardo,Roland Bassett,Austin C Cao,Dongfeng Tan

Frontiers in oncology 11:719564 PubMed34722263

2021

Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Su,Yufang Lv,Wenhao Lu,Zhenyuan Yu,Yu Ye,Bingqian Guo,Deyun Liu,Haibiao Yan,Tianyu Li,Qingyun Zhang,Jiwen Cheng,Zengnan Mo

Neoplasma 68:702-710 PubMed33884883

2021

SPOCK1 promotes the proliferation and migration of colon cancer cells by regulating the NF-κB pathway and inducing EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Xiong Liu,Yuan-Yuan Cao,Jia-Yao Qu

Bioengineered 12:578-588 PubMed33517826

2021

Long non-coding RNA TTN antisense RNA 1 facilitates hepatocellular carcinoma progression via regulating miR-139-5p/SPOCK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghao Zhu,Shiqing Jiang,Zongyao Wu,Tonghua Liu,Wei Zhang,Lili Wu,Lijun Xu,Mingliang Shao

Cancer biotherapy & radiopharmaceuticals : PubMed33090888

2020

Knockdown of Long Noncoding RNA Represses Gallbladder Cancer Advancement by Regulating Expression Through Sponging .

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Lijian Chen,Xundi Xu,Chao Shen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPOCK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (AB229935)

Western blot - Anti-SPOCK1 antibody (AB229935)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise