Rabbit Polyclonal SPOCK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Testican-1 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Expected | Tested | Not recommended |
Mouse | Tested | Expected | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Select an associated product type
May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.
SPOCK, TIC1, TICN1, TIC1, TICN1, SPOCK, SPOCK1, Testican-1, Protein SPOCK
Rabbit Polyclonal SPOCK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Testican-1 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein G
Purity >95%.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
SPOCK1 also known as Testican-1 is a proteoglycan involved mechanically in the regulation of cell-matrix interactions. It's a protein of approximately 54 kDa mass. SPOCK1 is expressed in various tissues with significant expression in the brain and skeletal muscle. In the extracellular matrix it forms part of the calcium-binding protein family having an active role in modulating matrix metalloproteinases (MMPs) and serine protease activities.
SPOCK1 influences cellular adhesion migration and communication. It interacts with several extracellular matrix components participating actively in the structural organization of tissues. SPOCK1 often associates with certain complexes binding to proteins like fibronectin and laminin which are important for tissue integrity. Its function can impact cell signaling and tissue remodeling reflecting its diverse biological roles.
SPOCK1 plays significant roles in the Wnt signaling pathway and the tissue inhibitors of metalloproteinases (TIMPs) pathway. In these pathways SPOCK1 modulates activities of proteins such as MMP2 and MMP9 which are pivotal for extracellular matrix degradation and remodeling. Its interaction with Wnt proteins highlights SPOCK1's influence in developmental and disease processes requiring precise cellular communication.
SPOCK1 links to cancers and fibrotic diseases. In cancer its overexpression often correlates with tumor progression and metastasis interacting with proteins like integrins to facilitate these processes. In fibrosis altered SPOCK1 levels affect extracellular matrix composition and deposition in conjunction with proteins like collagen contributing to tissue scarring and dysfunction. Understanding SPOCK1's roles provides insight into these conditions' pathology.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Lane 1: 293T whole cell lysate.
ab229935 antibody at 1:2000 dilution.
Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 50 kDa Observed band size: 50 kDa.
All lanes: Western blot - Anti-SPOCK1 antibody (ab229935)
Predicted band size: 49 kDa
All lanes: Western blot - Anti-SPOCK1 antibody (ab229935) at 1/2000 dilution
All lanes: Mouse brain tissue lysate
All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Paraffin-embedded human kidney tissue stained for SPOCK1 using ab229935 at 1/100 dilution in immunohistochemical analysis.
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