Anti-SPOCK2 antibody

• WB

Supplier Data

Western blot - Anti-SPOCK2 antibody (AB217044)

All lanes:

Western blot - Anti-SPOCK2 antibody (ab217044) at 1/300 dilution

All lanes:

HeLa cell lysate

Secondary

All lanes:

Anti-rabbit antibody at 1/10000 dilution

Predicted band size: 47 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK2 antibody (AB217044)

Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat brain tissue labeling SPOCK2 with ab217044 at 1/200 dilution followed by a conjugated secondary and DAB staining.

• WB

CiteAb

Western blot - Anti-SPOCK2 antibody (AB217044)

SPOCK2 western blot using anti-SPOCK2 antibody ab217044. Publication image and figure legend from Ge, P. L., Li, S. F., et al., 2020, Aging (Albany NY), PubMed 32213661.

ab217044 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab217044 please see the product overview.

Measurement of TMC8, BIN2 and SPOCK2 at mRNA and protein level in our cohort. (A, B) Relative mRNA levels of TMC8 and BIN2 in 10 HCC samples were both overexpressed compared with matched normal samples by qRT-PCR. (C) Representative western blot images showed protein expression of TMC8, BIN2 and SPOCK2 were overexpressed in HCC tissue than those in normal liver tissue. (D–F) Western blot analysis demonstrated that mean greyscale of TMC8, BIN2 and SPOCK2 were all higher in 10 fresh-frozen HCC tissues compared with those in matched adjacent normal liver tissues. (G) Representative immunohistochemical staining images of TMC8, BIN2 and SPOCK2, which were all mainly expressed in the cytoplasm. Original magnification : x200. (H–J) Mean protein expression of TMC8, BIN2 and SPOCK2 in 35 HCC were all significantly higher compared with those in adjacent non-tumour tissue by immunohistochemistry. MAD : mean areal density.

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