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Rabbit Polyclonal SPON2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPON2 aa 100-250.

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Images

Western blot - Anti-SPON2 antibody (AB221649), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SPON2 antibody (AB221649), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SPON2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUD6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
0.04000-0.40000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
0.25000-2.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100.

Associated Products

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2 products for Alternative Product

Target data

Function

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).

Alternative names

Recommended products

Rabbit Polyclonal SPON2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPON2 aa 100-250.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human SPON2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUD6
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SPON2 antibody (ab221649), expandable thumbnail

    Western blot - Anti-SPON2 antibody (ab221649)

    All lanes: Western blot - Anti-SPON2 antibody (ab221649) at 1/250 dilution

    Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate

    Lane 2: U-251 MG (human brain glioma cell line) cell lysate

    Lane 3: Human plasma

    Lane 4: Human liver lysate

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SPON2 antibody (ab221649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SPON2 antibody (ab221649)

    PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SPON2 (green) using ab221649 at 4 μg/ml in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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