Rabbit Recombinant Monoclonal SPON2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Human samples.
Images
![Western blot - Anti-SPON2 antibody [EPR25828A-87] (AB302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--western-blot-img404138.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPON2 antibody [EPR25828A-87] (AB302897)")
![Flow Cytometry (Intracellular) - Anti-SPON2 antibody [EPR25828A-87] (AB302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--flow-cytometry-intracellular-img403940.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SPON2 antibody [EPR25828A-87] (AB302897)")
![Immunocytochemistry - Anti-SPON2 antibody [EPR25828A-87] (AB302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--immunocytochemistry-img403903.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-SPON2 antibody [EPR25828A-87] (AB302897)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | I-ELISA | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).
Alternative names
Spondin-2, Differentially expressed in cancerous and non-cancerous lung cells 1, Mindin, DIL-1, UNQ435/PRO866, SPON2, DIL1
Recommended products
Rabbit Recombinant Monoclonal SPON2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Human samples.
Alternative names
Spondin-2, Differentially expressed in cancerous and non-cancerous lung cells 1, Mindin, DIL-1, UNQ435/PRO866, SPON2, DIL1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR25828A-87
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SPON2 antibody [EPR25828A-87] (ab302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--western-blot-img404138.jpg "Western blot - Anti-SPON2 antibody [EPR25828A-87] (ab302897)")
Western blot - Anti-SPON2 antibody [EPR25828A-87] (ab302897)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: PC-3, DU 145 (PMID: 22615945 )
Lysate in lane1 was freshly made and used for Western blotting immediately to minimize protein degradation. The band beneath the target band (40kDa) in Lane 2 is likely to be degraded target fragments.
Exposure time:
Lane 1: 81 seconds
Lane 2-4: 37 secondsAll lanes: Western blot - Anti-SPON2 antibody [EPR25828A-87] (ab302897) at 1/1000 dilution
Lane 1: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: LNCaP whole cell lysate at 20 µg
Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4: DU145 (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa
![Flow Cytometry (Intracellular) - Anti-SPON2 antibody [EPR25828A-87] (ab302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--flow-cytometry-intracellular-img403940.jpg "Flow Cytometry (Intracellular) - Anti-SPON2 antibody [EPR25828A-87] (ab302897)")
Flow Cytometry (Intracellular) - Anti-SPON2 antibody [EPR25828A-87] (ab302897)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell, Left) / LNCaP (human prostate carcinoma epithelial cell, Right) cells labelling SPON2 with ab302897 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression: PC-3 (PMID: 22615945).
![Immunocytochemistry - Anti-SPON2 antibody [EPR25828A-87] (ab302897), expandable thumbnail](https://content.abcam.com/products/images/spon2-antibody-epr25828a-87-ab302897--immunocytochemistry-img403903.jpg "Immunocytochemistry - Anti-SPON2 antibody [EPR25828A-87] (ab302897)")
Immunocytochemistry - Anti-SPON2 antibody [EPR25828A-87] (ab302897)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling SPON2 with ab302897 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing mainly cytoplasmic staining in LNCaP cell line and weak staining in PC-3 cell line. Low expression: PC-3 (PMID: 22615945) is observed.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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