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Rabbit Polyclonal SPOP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOP aa 1-250.


Images

Western blot - Anti-SPOP antibody (AB137537), expandable thumbnail
  • Western blot - Anti-SPOP antibody (AB137537), expandable thumbnail
  • Western blot - Anti-SPOP antibody (AB137537), expandable thumbnail
  • Western blot - Anti-SPOP antibody (AB137537), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SPOP aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43791

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Predicted
Cow
Predicted
Xenopus tropicalis
Predicted
Zebrafish
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Rat, Cow, Zebrafish, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability (PubMed:32109420). Plays an essential role for proper translation, but not for their degradation, of critical DNA replication licensing factors CDT1 and CDC6, thereby participating in DNA synthesis and cell proliferation (PubMed:36791496). Regulates interferon regulatory factor 1/IRF1 proteasomal turnover by targeting S/T-rich degrons in IRF1 (PubMed:37622993). Facilitates the lysosome-dependent degradation of enterovirus EV71 protease 2A by inducing its 'Lys-48'-linked polyubiquitination, which ultimately restricts EV71 replication (PubMed:37796126). Acts as an antiviral factor also against hepatitis B virus/HBV by promoting ubiquitination and subsequent degradation of HNF1A (PubMed:38018242). In turn, inhibits HBV transcription and replication by preventing HNF1A stimulating activity of HBV preS1 promoter and enhancer II (PubMed:38018242).

Alternative names

Recommended products

Rabbit Polyclonal SPOP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOP aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SPOP aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43791
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-SPOP antibody (ab137537), expandable thumbnail

    Western blot - Anti-SPOP antibody (ab137537)

    10% SDS-PAGE

    All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/1000 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Lane 2: HepG2 nuclear extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 42 kDa

  • Western blot - Anti-SPOP antibody (ab137537), expandable thumbnail

    Western blot - Anti-SPOP antibody (ab137537)

    10% SDS PAGE

    All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/2000 dilution

    All lanes: Mouse brain lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 42 kDa

  • Western blot - Anti-SPOP antibody (ab137537), expandable thumbnail

    Western blot - Anti-SPOP antibody (ab137537)

    10% SDS-PAGE

    All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/5000 dilution

    Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: Transfected HEK-293T whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 42 kDa

  • Western blot - Anti-SPOP antibody (ab137537), expandable thumbnail

    Western blot - Anti-SPOP antibody (ab137537)

    Western blot: Anti-SPOP antibody (ab137537) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137537 was shown to bind specifically to SPOP. A band was observed at 42 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in SPOP knockout cell line ab277845. To generate this image, wild-type and SPOP knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: SPOP knockout HEK-293 cell lysate at 20 µg

    Lane 3: Recombinant Human SPOP protein Recombinant Human SPOP protein ab105599 cell lysate at 0.1 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 42 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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