Rabbit Polyclonal SPOP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOP aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Predicted |
Cow | Predicted |
Xenopus tropicalis | Predicted |
Zebrafish | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
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Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability (PubMed:32109420). Plays an essential role for proper translation, but not for their degradation, of critical DNA replication licensing factors CDT1 and CDC6, thereby participating in DNA synthesis and cell proliferation (PubMed:36791496). Regulates interferon regulatory factor 1/IRF1 proteasomal turnover by targeting S/T-rich degrons in IRF1 (PubMed:37622993). Facilitates the lysosome-dependent degradation of enterovirus EV71 protease 2A by inducing its 'Lys-48'-linked polyubiquitination, which ultimately restricts EV71 replication (PubMed:37796126). Acts as an antiviral factor also against hepatitis B virus/HBV by promoting ubiquitination and subsequent degradation of HNF1A (PubMed:38018242). In turn, inhibits HBV transcription and replication by preventing HNF1A stimulating activity of HBV preS1 promoter and enhancer II (PubMed:38018242).
Speckle-type POZ protein, HIB homolog 1, Roadkill homolog 1, SPOP
Rabbit Polyclonal SPOP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPOP aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Terms & Conditions.
10% SDS-PAGE
All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/1000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Lane 2: HepG2 nuclear extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa
10% SDS PAGE
All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/2000 dilution
All lanes: Mouse brain lysate at 50 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa
10% SDS-PAGE
All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/5000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Transfected HEK-293T whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa
Western blot: Anti-SPOP antibody (ab137537) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137537 was shown to bind specifically to SPOP. A band was observed at 42 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in SPOP knockout cell line ab277845. To generate this image, wild-type and SPOP knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-SPOP antibody (ab137537) at 1/1000 dilution
Lane 1: Wild-type HEK-293 cell lysate at 20 µg
Lane 2: SPOP knockout HEK-293 cell lysate at 20 µg
Lane 3: Recombinant Human SPOP protein Recombinant Human SPOP protein ab105599 cell lysate at 0.1 µg
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 42 kDa
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