AB190253

Anti-SPP antibody - C-terminal

Name: Anti-SPP antibody - C-terminal (ab190253) | Abcam
Brand: Abcam Limited
SKU: AB190253
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal SPP antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human HM13 aa 300 to C-terminus.

View Alternative Names

H13, IMP1, PSL3, SPP, MSTP086, HM13, Minor histocompatibility antigen H13, Intramembrane protease 1, Presenilin-like protein 3, Signal peptide peptidase, IMP-1, IMPAS-1, hIMP1

2 Images

Supplier Data

Immunoprecipitation - Anti-SPP antibody - C-terminal (AB190253)

Detection of SPP in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab190253 at 6 μg/reaction for IP and 1 μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-SPP antibody - C-terminal (ab190253)

Predicted band size: 41 kDa

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Supplier Data

Western blot - Anti-SPP antibody - C-terminal (AB190253)

All lanes:

Western blot - Anti-SPP antibody - C-terminal (ab190253) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 41 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HM13 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TCT9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab190253 was affinity purified using an epitope specific to SPP immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein, resulting in the release of the fragment from the ER membrane into the cytoplasm. Required to generate lymphocyte cell surface (HLA-E) epitopes derived from MHC class I signal peptides (PubMed : 11714810). May be necessary for the removal of the signal peptide that remains attached to the hepatitis C virus core protein after the initial proteolytic processing of the polyprotein (PubMed : 12145199). Involved in the intramembrane cleavage of the integral membrane protein PSEN1 (PubMed : 11714810, PubMed : 12077416, PubMed : 14741365). Cleaves the integral membrane protein XBP1 isoform 1 in a DERL1/RNF139-dependent manner (PubMed : 25239945). May play a role in graft rejection (By similarity).

See full target information HM13

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:199 PubMed40399808

2025

Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxuan Wang,Yanyan Lin,Qianqian Zhao,Huanliang Chen,Shisuo Du,Zhaochong Zeng

Histology and histopathology 40:1435-1446 PubMed39703990

2024

HM13 is a predictive biomarker of metastasis and neutrophil infiltration in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbing Ren,Ying Mao,Xiao Yuan

Journal of cell science 137: PubMed39513424

2024

Topology surveillance of the lanosterol demethylase CYP51A1 by signal peptide peptidase.

Applications

Unspecified application

Species

Unspecified reactive species

Nikita Sergejevs,Dönem Avci,Michael L van de Weijer,Robin A Corey,Marius K Lemberg,Pedro Carvalho

Journal of virology 98:e0120624 PubMed39207137

2024

Regulation of the cell surface expression of classical and non-classical MHC proteins by the human cytomegalovirus UL40 and rhesus cytomegalovirus Rh67 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Brackenridge,Nessy John,Wanlin He,Klaus Früh,Persephone Borrow,Andrew McMichael

Cell death & disease 12:738 PubMed34312365

2021

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwei Luo,Jingyan Li,Ziqing Li,Tong Lin,Lili Zhang,Wanqi Yang,Yanqi Mai,Ruiming Liu,Meiting Chen,Chunmei Dai,Hanwei Yang,Jing Lu,Hong Li,Guimei Guan,Min Huang,Peiqing Liu,Zhuoming Li

Molecular cell 79:768-781.e7 PubMed32738194

2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L van de Weijer,Logesvaran Krshnan,Sabrina Liberatori,Elena Navarro Guerrero,Jacob Robson-Tull,Lilli Hahn,Robert Jan Lebbink,Emmanuel J H J Wiertz,Roman Fischer,Daniel Ebner,Pedro Carvalho

EMBO reports 19: PubMed29519897

2018

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Stefanovic-Barrett,Anna S Dickson,Stephen P Burr,James C Williamson,Ian T Lobb,Dick Jh van den Boomen,Paul J Lehner,James A Nathan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SPP antibody - C-terminal

Immunoprecipitation - Anti-SPP antibody - C-terminal (AB190253)

Western blot - Anti-SPP antibody - C-terminal (AB190253)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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