Anti-SPRED1 antibody [EPR24412-120] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-SPRED1 antibody [EPR24412-120] - BSA and Azide free (AB282019)

This data was developed using ab271191, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling SPRED1 with ab271191 at 1/500 dilution (0.1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• WB

Lab

Western blot - Anti-SPRED1 antibody [EPR24412-120] - BSA and Azide free (AB282019)

This data was developed using ab271191, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration :

3 minutes

Exposure time :

All lanes:

Western blot - Anti-SPRED1 antibody [EPR24412-120] (<a href='/en-us/products/primary-antibodies/spred1-antibody-epr24412-120-ab271191'>ab271191</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lane 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

false

• WB

Lab

Western blot - Anti-SPRED1 antibody [EPR24412-120] - BSA and Azide free (AB282019)

This data was developed using ab271191, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-SPRED1 antibody [EPR24412-120] (<a href='/en-us/products/primary-antibodies/spred1-antibody-epr24412-120-ab271191'>ab271191</a>) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with SPRED1 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

false

• WB

Lab

Western blot - Anti-SPRED1 antibody [EPR24412-120] - BSA and Azide free (AB282019)

This data was developed using ab271191, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : skeletal muscle, liver and testis (PMID : 15580519, 15630138).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SPRED1 antibody [EPR24412-120] (<a href='/en-us/products/primary-antibodies/spred1-antibody-epr24412-120-ab271191'>ab271191</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false